ScholarWorks Community:https://scholarworks.korea.ac.kr/kumedicine/handle/2020.sw.kumedicine/32024-03-28T12:45:35Z2024-03-28T12:45:35ZTranscriptomic data of human adrenocortical NCI-H295R cells treated with cortisol biosynthesis inhibitorsKim, Soo HyunKim, Hyun JungJung, Jong-WhaChung, SooyoungSon, Gi Hoonhttps://scholarworks.korea.ac.kr/kumedicine/handle/2021.sw.kumedicine/654742024-02-22T01:00:06Z2024-02-01T00:00:00ZTitle: Transcriptomic data of human adrenocortical NCI-H295R cells treated with cortisol biosynthesis inhibitors
Authors: Kim, Soo Hyun; Kim, Hyun Jung; Jung, Jong-Wha; Chung, Sooyoung; Son, Gi Hoon
Abstract: Adrenal corticosteroid biosynthesis dysregulation can give rise to various pathological conditions, such as Cushing's syndrome, a disorder characterized by the sustained and excessive production of cortisol. Despite the development of several classes of steroidogenesis inhibitors to treat human diseases associated with cortisol overproduction, their use is limited by insufficient efficacy, adverse effects, and/or tolerability. Recently, we identified a series of benzimidazolylurea derivatives, including the representative compound CJ28, as novel cortisol biosynthesis inhibitors [1l . They significantly inhibited both basal and stimulated production of cortisol in NCI-H295R cells, a human adrenocarcinoma cell line. The inhibitory effects were attributed to both attenuated steroidogenesis and de novo cholesterol biosynthesis. Here, we provide transcriptomic (RNA-seq) data from adrenal cell cultures in response to treatment with either CJ28 or metyrapone (MET), an inhibitor of 11 beta-hydroxylase). Total RNA was extracted from the cells treated with vehicle (0.1% DMSO), CJ28 (30 mu M), or MET (30 mu M) for 24 h. Primary sequence data were acquired using paired-end sequencing on an Illumina NovaSeq 60 0 0 platform. The raw RNA-seq data have been deposited in the Gene Expression Omnibus (GEO) database (GSE236435). This dataset is a useful resource for providing valuable information on the gene expression networks underlying adrenocortical steroidogenesis.(c) 2023 Published by Elsevier Inc. This is an open access article under the CC BY license ( http://creativecommons.org/licenses/by/4.0/ )2024-02-01T00:00:00ZThree-dimensional imaging and quantification of epidermal innervation in atopic dermatitisKim, D.Lee, S.Seo, S.Ahn, H.Sun, W.Kim, B.Rhyu, I.https://scholarworks.korea.ac.kr/kumedicine/handle/2021.sw.kumedicine/640652023-12-27T01:01:59Z202305-01-01T00:00:00ZTitle: Three-dimensional imaging and quantification of epidermal innervation in atopic dermatitis
Authors: Kim, D.; Lee, S.; Seo, S.; Ahn, H.; Sun, W.; Kim, B.; Rhyu, I.
Abstract: Understanding the structural alteration of epidermal innervation is essential for a deeper understanding of neurogenic inflammation and pruritus in atopic dermatitis. The skin of atopic dermatitis patients has been reported to show controversial results, higher or lower intraepidermal nerve fiber densities (IENFDs). Therefore, this study was intended to verify the genuine structural alteration of epidermal innervation in atopic dermatitis through the skin-clearing technique and related three-dimensional (3-D) image quantification process as previously reported. A total of 10 (five healthy and five atopic dermatitis subjects) participants were prospectively enrolled. All subjects were male, 20s, and received biopsy/suction blister on a similar anatomical site, proximal forearm, to control other variables which could affect the results of IENFDs. The atopic dermatitis patients displayed significantly decreased intraepidermal nerve fibers, nodes, end, total length, total volume, and complexity compared with the results of healthy subjects. Furthermore, the arborizing intermingled structures of healthy subjects represented by nodes and complexity seemed to be more vividly simplified in the atopic dermatitis patients compared with the degree of decrease of well-known parameters, number of nerve fibers, or total length. In conclusion, our study successfully demonstrated the difference of 3-D whole structural images of epidermal innervation between healthy subjects and atopic dermatitis patients. Further studies with a larger number of participants are expected to show the possible relationship among structural alteration of epidermal innervation, neurosensory symptoms, and neurogenic inflammation of atopic dermatitis.202305-01-01T00:00:00ZPlate-curving cell culture as a model for assessing curvature-induced cellular responses during neural tube morphogenesisAhn, Hyung-MinSun, Woonghttps://scholarworks.korea.ac.kr/kumedicine/handle/2021.sw.kumedicine/649642024-01-04T00:00:07Z2023-12-01T00:00:00ZTitle: Plate-curving cell culture as a model for assessing curvature-induced cellular responses during neural tube morphogenesis
Authors: Ahn, Hyung-Min; Sun, Woong
Abstract: Neurulation is an important shape-transforming event during embryonic development where a flat neural plate is converted into a neural tube. Failure in this morphogenetic process accounts for one of the most common birth defects. Mechanical biology has provided key insights into neural tube formation and curvature among many physical properties that are eliciting attention. However, the lack of a proper model to study the effect of curvature has limited the potential to reveal its role in neurulation. In this study, we introduce a novel cell culture method called plate-curving cell culture where a polydimethylsiloxane (PDMS) plate of desired physical properties is curved in either a concave or convex form while the human pluripotent stem cell culture induced to have early neural plate identity is placed on top of its surface. With this method, we observed the elongation of cell colony morphology, as well as the perpendicular alignment of the cell division axis in the concave surface; the oriented cell division does not seem to explain the colony elongation. Transcriptome comparison in search of alternate possibilities suggested selectively altered pathways in the concave surface culture. Our new method is widely available, easy-to-use and culture-friendly, facilitating future mechanobiological studies of neurulation.2023-12-01T00:00:00ZMitochondrial E3 ligase MARCH5 is a safeguard against DNA-PKcs-mediated immune signaling in mitochondria-damaged cellsHeo, JunePark, Yeon-JiKim, YonghyeonLee, Ho-SooKim, JeongahKwon, Soon-HwanKang, Myeong-GyunRhee, Hyun-WooSun, WoongLee, Jae-HoCho, Hyeseonghttps://scholarworks.korea.ac.kr/kumedicine/handle/2021.sw.kumedicine/649702024-01-04T00:30:05Z2023-12-01T00:00:00ZTitle: Mitochondrial E3 ligase MARCH5 is a safeguard against DNA-PKcs-mediated immune signaling in mitochondria-damaged cells
Authors: Heo, June; Park, Yeon-Ji; Kim, Yonghyeon; Lee, Ho-Soo; Kim, Jeongah; Kwon, Soon-Hwan; Kang, Myeong-Gyun; Rhee, Hyun-Woo; Sun, Woong; Lee, Jae-Ho; Cho, Hyeseong
Abstract: Mitochondrial dysfunction is important in various chronic degenerative disorders, and aberrant immune responses elicited by cytoplasmic mitochondrial DNA (mtDNA) may be related. Here, we developed mtDNA-targeted MTERF1-FokI and TFAM-FokI endonuclease systems to induce mitochondrial DNA double-strand breaks (mtDSBs). In these cells, the mtDNA copy number was significantly reduced upon mtDSB induction. Interestingly, in cGAS knockout cells, synthesis of interferon beta 1 and interferon-stimulated gene was increased upon mtDSB induction. We found that mtDSBs activated DNA-PKcs and HSPA8 in a VDAC1-dependent manner. Importantly, the mitochondrial E3 ligase MARCH5 bound active DNA-PKcs in cells with mtDSBs and reduced the type CYRILLIC CAPITAL LETTER BYELORUSSIAN-UKRAINIAN I interferon response through the degradation of DNA-PKcs. Likewise, mitochondrial damage caused by LPS treatment in RAW264.7 macrophage cells increased phospho-HSPA8 levels and the synthesis of mIFNB1 mRNA in a DNA-PKcs-dependent manner. Accordingly, in March5 knockout macrophages, phospho-HSPA8 levels and the synthesis of mIFNB1 mRNA were prolonged after LPS stimulation. Together, cytoplasmic mtDNA elicits a cellular immune response through DNA-PKcs, and mitochondrial MARCH5 may be a safeguard to prevent persistent inflammatory reactions.2023-12-01T00:00:00Z