https://scholarworks.korea.ac.kr/kumedicine/handle/2020.sw.kumedicine/63 Thu, 09 Apr 2026 16:38:38 GMT 2026-04-09T16:38:38Z https://scholarworks.korea.ac.kr/kumedicine/handle/2021.sw.kumedicine/78864 Title: Preparation of bone marrow-derived mast cells capable of producing high levels of histamine comparable to mature mast cells in vivo Authors: Sur, Bongjun; Shin, Kyungsook; Cho, Sung-Weon Abstract: Mast cells are the crucial immune cells responsible for the production of histamine and diverse cytokines. Immature mast cells were prepared conventionally by culturing bone marrow cells (BMC) for culturing using low concentrations of IL-3 (5 ng/ml) and SCF (5 ng/ml) to produce the histamine level much lower than that of mature mast cells in vivo. The cells of rat bone marrow derived mast cells (rBMMCs) were studied for histamine production by culturing with high cytokine concentrations (IL-3: 50 ng/ml, SCF: 50 ng/ml) and high cell densities (5 x 105/ml). The cells were applied to transcriptome, real time PCR, and ELISA. The cells were analyzed for the effect of LPS on histamine production. rBMMCs cultured in conditions were measured to produce histamine reaching up to 25 ng/cell, comparable to the mature mast cells. The cells were observed to produce various cytokines and receptors in transcriptome analysis, The cells were analyzed to express the increase by 4.2-folds in HDC, by 870-folds in HRH1, by 2.7-fold in TLR4, and by 27-fold in TLR7 expression compared to non-cultured BMC in real time PCR. The histamine production of cells was increased by 2 with LPS. The cells were to produce TNF-α affected by cytokine concentration, measured via ELISA. These findings indicated that rat BMMCs are the good model for mast cell in vitro to produce the high level histamine comparable to mature mast cells in vivo, to respond to LPS and to exhibit elevated TLR4 and TNF-α production. Tue, 06 May 2025 00:00:00 GMT https://scholarworks.korea.ac.kr/kumedicine/handle/2021.sw.kumedicine/78864 2025-05-06T00:00:00Z https://scholarworks.korea.ac.kr/kumedicine/handle/2021.sw.kumedicine/65253 Title: The induction of histamine synthesis from rat bone marrow derived mast cells with rat stem cell factor Authors: Cho, Sung-Weon; Sur, Bong Jung; Shin, Kyung Sook Abstract: Mast cells are the main cells to produce histamine regarded as the main factor to induce allergy. To comprehend the allergy by mast cell activation, The mast cells of bone marrow derived mast cells (BMMC) are usually prepared by culturing bone marrow cells including rodent hematopoietic stem cells, in the presence of both IL-3 and stem cell factor (SCF). However, BMMC was reported not to produce the high levels histamine comparable to mature mast cells in host tissue. In this study, rat BMMC (rBMMC) were cultured in 96 well plate to induce diverse conditions in cell numbers per unit culture media, rat IL-3 and rat SCF to find the conditions to synthesize high level histamine. Cells of rat bone marrow cells were cultured in the cell density of 1.1×10 5/ml (low density, lowD) and 5.5 × 10 5/ml (high D), rat IL-3 was added to the levels of 10 ng/ml (low 3) or 50 ng/ml (high 3), Rat SCF was adjusted to the levels of 12.5 (lows), 25 (midS) and 50 (highS) ng/ml. Histamine included in 5×10 4BMMC were extracted by perchloric acid, n-butanol, and heptane. The levels of histamine were measured by ophthalialdehyde solution and read for fluorometric intensity. The levels at culture day 4 (D 4) were in the range of 12~45 ng. The significant elevation was observed at D 11 in the conditions of lowD high3 (153 ng), however, D 8 in highD high3 (253 ng). The average histamine level was 74 ng in the condition of low Dlow 3, but 112 ng in low Dhigh 3 at D 11. That was 106 ng in high Dlow 3 but 183 ng in high Dhigh 3 at culture D 8. These results indicated that rat IL-3 increased the level of histamine synthesis and shorten the histamine production from undifferentiated hematopoietic stem cells. Sun, 01 Jan 202305 00:00:00 GMT https://scholarworks.korea.ac.kr/kumedicine/handle/2021.sw.kumedicine/65253 202305-01-01T00:00:00Z https://scholarworks.korea.ac.kr/kumedicine/handle/2020.sw.kumedicine/28172 Title: Effectiveness of Mass Drug Administration on Neglected Tropical Diseases in Schoolchildren in Zanzibar, Tanzania Authors: Kim, Ju Yeong; Sim, Seobo; Chung, Eun Joo; Rim, Han-Jong; Chai, Jong-Yil; Min, Duk-Young; Eom, Keeseon S.; Mohammed, Khalfan A.; Khamis, Iddi S.; Yong, Tai-Soon Abstract: Soil-transmitted helminths and Schistosoma haematobium affect more than 3 billion people globally and mainly occur in sub-Saharan Africa. The present study assessed the overall infection status of a 1716-student cohort of school-children in Zanzibar and applied mass drug administration (MDA) to the cohort from 2007 to 2009. Schools in Pemba, Zanzibar, had a much higher prevalence of soil-transmitted helminth infections than those in Unguja, and the Chaani, Ghana, and Machui schools of Unguja exhibited high S. haematobium infection rates. The MDA program only partially controlled parasite infections, owing to high rates of re-infection. The infection rate of S. haematobium across all 10 schools, for example, was only reduced by 1.8%, and even this change not significant, even though the S. haematobium infection rates of the Chaani and Mzambarauni schools were significantly reduced from 64.4 and 23.4%, respectively, at the first screening, to 7.3 and 2.3% at the last screening. The overall infection rate of Ascaris lumbricoides was reduced from 36.0% at the first screening to 22.6% at the last screening. However, the infection rates for both Trichuris trichiura and hookworm were generally unaffected by MDA. In the future, parasite control programs should involve strategically designed MDA schedules and holistic intervention (e.g., sanitation improvement, hygiene behavior changes, and control of intermediated hosts). Wed, 01 Apr 2020 00:00:00 GMT https://scholarworks.korea.ac.kr/kumedicine/handle/2020.sw.kumedicine/28172 2020-04-01T00:00:00Z https://scholarworks.korea.ac.kr/kumedicine/handle/2020.sw.kumedicine/33092 Title: Differential synapse density between Purkinje cell dendritic spine and parallel fiber varicosity in the rat cerebellum among the phylogenic lobules Authors: Kim, Hyun Wook; Oh, Seung Hak; Lee, Se Jeong; Na, Ji eun; Rhyu, Im Joo Abstract: The cerebellum is a region of the brain that plays an important role in motor control. It is classified phylogenetically into archicerebellum, paleocerebellum and neocerebellum. The Purkinje cells are lined in a row called Purkinje cell layer and it has a unique dendritic branches with many spines. The previous study reported that there is a difference of synapse density according to the lobules based on large two-dimensional data. However, recent study with high voltage electron microscopy showed there was no differences in dendritic spine density of the Purkinje cell according to its phylogenetic lobule. We analyzed Purkinje cell density in the II, VI and X lobules by stereological modules and synaptic density was estimated by double disector based on Purkinje cell density in the molecular layer of each lobule. The results showed that there was significant difference in the Purkinje cell density and synapse number according to their phylogenetic lobules. The number of Purkinje cell in a given volume was larger in the archicerebellum, but synapse density was higher in the neocerebellum. These data suggest that cellular and synaptic organization of the Purkinje cell is different according to their phylogenetic background. Sat, 01 Feb 2020 00:00:00 GMT https://scholarworks.korea.ac.kr/kumedicine/handle/2020.sw.kumedicine/33092 2020-02-01T00:00:00Z