Detailed Information

Cited 23 time in webofscience Cited 22 time in scopus
Metadata Downloads

Quantitative Estimation of the Total Sulfide Concentration in Live Tissues by Two-Photon Microscopy

Authors
Lim, Chang SuDas, Sajal KumarYang, Sun YoungKim, Eun SunChun, Hoon JaiCho, Bong Rae
Issue Date
1-Oct-2013
Publisher
AMER CHEMICAL SOC
Citation
ANALYTICAL CHEMISTRY, v.85, no.19, pp 9288 - 9295
Pages
8
Indexed
SCI
SCIE
SCOPUS
Journal Title
ANALYTICAL CHEMISTRY
Volume
85
Number
19
Start Page
9288
End Page
9295
URI
https://scholarworks.korea.ac.kr/kumedicine/handle/2020.sw.kumedicine/10254
DOI
10.1021/ac402042k
ISSN
0003-2700
1520-6882
Abstract
Hydrogen sulfide (H2S) is a newly recognized transmitter, which protects various organs from oxidative stress. In this article, we report a ratiometric two-photon probe, TFCA, which can be excited by 750 nm femtosecond pulses, shows a 110-fold increase in the intensity ratio upon reaction with HS- and high selectivity for HS- and can visualize the total sulfide ([H2S] + [HS-]) distribution in live tissue by two-photon microscopy (TPM). We also developed a kinetic method to quantitatively estimate the total sulfide concentration ([H2S] + [HS-]) in live tissues. The kinetic method allowed us to measure the observed rate constants (k(obs)) for the sulfide-induced deazidation reaction of TFCA in live cells and tissues using TPM. The total sulfide concentration was calculated by using k(obs) = k(2)[HS-], with the k(2) value determined in HEPES/EtOH (1/1, pH = 7.2), and [H2S]/[HS-] = [H+]/K-2. The total sulfide concentration was found to be nearly zero in HeLa cells and 4-7 mu M in rat colon tissues.
Files in This Item
There are no files associated with this item.
Appears in
Collections
2. Clinical Science > Department of Gastroenterology and Hepatology > 1. Journal Articles

qrcode

Items in ScholarWorks are protected by copyright, with all rights reserved, unless otherwise indicated.

Related Researcher

Researcher Chun, Hoon Jai photo

Chun, Hoon Jai
Anam Hospital (Department of Gastroenterology and Hepatology, Anam Hospital)
Read more

Altmetrics

Total Views & Downloads

BROWSE