Effects of urinary and recombinant gonadotropins on in vitro maturation outcomes of mouse preantral follicles
- Authors
- Park K.E.; Ku S.-Y.; Jung K.C.; Liu H.C.; Kim Y.Y.; Kim Y.J.; Kim S.H.; Choi Y.M.; Kim J.G.; Moon S.Y.
- Issue Date
- Aug-2013
- Publisher
- SAGE PUBLICATIONS INC
- Keywords
- in vitro maturation; ovarian stimulation; preantral follicles; recombinant gonadotropins; urinary gonadotropin
- Citation
- Reproductive Sciences, v.20, no.8, pp 909 - 916
- Pages
- 8
- Indexed
- SCI
SCIE
SCOPUS
- Journal Title
- Reproductive Sciences
- Volume
- 20
- Number
- 8
- Start Page
- 909
- End Page
- 916
- URI
- https://scholarworks.korea.ac.kr/kumedicine/handle/2020.sw.kumedicine/11239
- DOI
- 10.1177/1933719112468948
- ISSN
- 1933-7191
1933-7205
- Abstract
- Gonadotropins including follicle-stimulating hormone (FSH) and luteinizing hormone (LH) play a crucial role in human-assisted reproduction techniques. Despite wide use of recombinant gonadotropins in clinical practice, the efficacy of urinary gonadotropins and the dosage of LH component have not yet been elucidated. This study was designed to investigate the difference of follicle culture outcomes according to various compositions of gonadotropins during in vitro culture of mouse preantral follicles. Ovaries were obtained from the 14-day-old C57BL/6 mice, and preantral follicles were isolated and cultured in culture media supplemented with human menopausal gonadotropin (hMG) 200 mIU/mL (group 1), recombinant FSH and LH (rFSH + rLH) 200 mIU/mL each (group 2), rFSH 200 mIU/mL + rLH 100 mIU/mL (group 3), or rFSH 200 mIU/mL + rLH 20 mIU/mL (group 4). Follicle survival rate was significantly lower in group 4. Antral follicles in lower doses of LH (groups 3, 4) showed a statistically significant larger diameter and tended to have a higher antral formation rate. However, follicles in group 1 tended to have a higher oocyte maturation rate. Estradiol concentration from conditioned media from 2:1 FSH/LH (group 3) was significantly higher than those from 1:1 FSH/LH (group 2) or 10:1 FSH/LH (group 4). Half dose of rLH to rFSH facilitated upregulation of growth differentiation factor 9 (Gdf9) expression in granulosa cells when compared to 1:1 FSH/LH or 10:1 FSH/LH. Conclusively, recombinant gonadotropins provided a comparable condition to hMG, and half dose of rLH to rFSH seems to be more suitable for follicular development during in vitro culture. © 2012 The Author(s).
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Collections - 2. Clinical Science > Department of Obstetrics and Gynecology > 1. Journal Articles
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