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Cited 12 time in webofscience Cited 14 time in scopus
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Heat Shock Proteins and Autophagy in Rats with Cerulein-Induced Acute Pancreatitis

Authors
Kim, Jin NamLee, Hong SikRyu, Soo HyungKim, You SunMoon, Jeong SeopKim, Chang DuckChang, In YoubYoon, Sang Pill
Issue Date
Dec-2011
Publisher
EDITORIAL OFFICE GUT & LIVER
Keywords
Acute pancreatitis; Autophagy; Heat shock protein
Citation
GUT AND LIVER, v.5, no.4, pp.513 - 520
Indexed
SCIE
SCOPUS
KCI
OTHER
Journal Title
GUT AND LIVER
Volume
5
Number
4
Start Page
513
End Page
520
URI
https://scholarworks.korea.ac.kr/kumedicine/handle/2020.sw.kumedicine/12957
DOI
10.5009/gnl.2011.5.4.513
ISSN
1976-2283
Abstract
Background/Aims: Heat shock proteins (HSPs) protect rats from cerulein-induced acute pancreatitis (AP) by preventing the subcellular redistribution of cathepsin B and the activation of trypsinogen. Autophagy plays a critical role in the secretion of digestive enzymes and triggering of cerulein-induced AP via the colocalization of trypsinogen and lysosomes. Therefore, using a rat cerulein-induced AP model, we investigated whether HSPs prevent AP by regulating autophagy. Methods: Twelve hours after fed standard laboratory chow and water, the experimental groups (cerulein, water-immersion [WI]-cerulein and heat-shock [HS]-cerulein) and the control groups (control, WI, and HS) received one intraperitoneal injection of cerulein (50 mu g/kg) or saline, respectively. All of the rats were sacrificed at 6 hours after injection. The severity of the AP was assessed based on the serum amylase level and the histological and electron microscopy findings. Western blotting was also performed for HSP60/70 and LC3B-II. Results: WI and HS induced HSP60 and HSP70, respectively. The induced HSP60/70 effectively prevented the development of cerulein-induced AP. Autophagy developed in the rats with cerulein-induced AP and was documented by the expression of LC3-II and electron microscopy findings. The WI-stressed rats and HS-treated rats did not develop cerulein-induced autophagy. Conclusions: HSPs exert protective effects against cerulein-induced AP in rats by inhibiting autophagy. (Gut Liver 2011;5:513-520)
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Lee, Hong Sik
안암병원 (Department of Gastroenterology and Hepatology, Anam Hospital)
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