Heat Shock Proteins and Autophagy in Rats with Cerulein-Induced Acute Pancreatitis
DC Field | Value | Language |
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dc.contributor.author | Kim, Jin Nam | - |
dc.contributor.author | Lee, Hong Sik | - |
dc.contributor.author | Ryu, Soo Hyung | - |
dc.contributor.author | Kim, You Sun | - |
dc.contributor.author | Moon, Jeong Seop | - |
dc.contributor.author | Kim, Chang Duck | - |
dc.contributor.author | Chang, In Youb | - |
dc.contributor.author | Yoon, Sang Pill | - |
dc.date.available | 2020-11-03T01:45:52Z | - |
dc.date.issued | 2011-12 | - |
dc.identifier.issn | 1976-2283 | - |
dc.identifier.issn | 2005-1212 | - |
dc.identifier.uri | https://scholarworks.korea.ac.kr/kumedicine/handle/2020.sw.kumedicine/12957 | - |
dc.description.abstract | Background/Aims: Heat shock proteins (HSPs) protect rats from cerulein-induced acute pancreatitis (AP) by preventing the subcellular redistribution of cathepsin B and the activation of trypsinogen. Autophagy plays a critical role in the secretion of digestive enzymes and triggering of cerulein-induced AP via the colocalization of trypsinogen and lysosomes. Therefore, using a rat cerulein-induced AP model, we investigated whether HSPs prevent AP by regulating autophagy. Methods: Twelve hours after fed standard laboratory chow and water, the experimental groups (cerulein, water-immersion [WI]-cerulein and heat-shock [HS]-cerulein) and the control groups (control, WI, and HS) received one intraperitoneal injection of cerulein (50 mu g/kg) or saline, respectively. All of the rats were sacrificed at 6 hours after injection. The severity of the AP was assessed based on the serum amylase level and the histological and electron microscopy findings. Western blotting was also performed for HSP60/70 and LC3B-II. Results: WI and HS induced HSP60 and HSP70, respectively. The induced HSP60/70 effectively prevented the development of cerulein-induced AP. Autophagy developed in the rats with cerulein-induced AP and was documented by the expression of LC3-II and electron microscopy findings. The WI-stressed rats and HS-treated rats did not develop cerulein-induced autophagy. Conclusions: HSPs exert protective effects against cerulein-induced AP in rats by inhibiting autophagy. (Gut Liver 2011;5:513-520) | - |
dc.format.extent | 8 | - |
dc.language | 영어 | - |
dc.language.iso | ENG | - |
dc.publisher | EDITORIAL OFFICE GUT & LIVER | - |
dc.title | Heat Shock Proteins and Autophagy in Rats with Cerulein-Induced Acute Pancreatitis | - |
dc.type | Article | - |
dc.publisher.location | 대한민국 | - |
dc.identifier.doi | 10.5009/gnl.2011.5.4.513 | - |
dc.identifier.scopusid | 2-s2.0-81855216293 | - |
dc.identifier.wosid | 000297428100017 | - |
dc.identifier.bibliographicCitation | GUT AND LIVER, v.5, no.4, pp 513 - 520 | - |
dc.citation.title | GUT AND LIVER | - |
dc.citation.volume | 5 | - |
dc.citation.number | 4 | - |
dc.citation.startPage | 513 | - |
dc.citation.endPage | 520 | - |
dc.type.docType | Article | - |
dc.identifier.kciid | ART001626894 | - |
dc.description.isOpenAccess | N | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
dc.description.journalRegisteredClass | kciCandi | - |
dc.relation.journalResearchArea | Gastroenterology & Hepatology | - |
dc.relation.journalWebOfScienceCategory | Gastroenterology & Hepatology | - |
dc.subject.keywordPlus | TRYPSINOGEN ACTIVATION | - |
dc.subject.keywordPlus | ACINAR-CELLS | - |
dc.subject.keywordPlus | STRESS-PROTEINS | - |
dc.subject.keywordPlus | INVOLVEMENT | - |
dc.subject.keywordPlus | CHAPERONES | - |
dc.subject.keywordPlus | EXPRESSION | - |
dc.subject.keywordPlus | PROTECTION | - |
dc.subject.keywordPlus | DISEASE | - |
dc.subject.keywordPlus | MICE | - |
dc.subject.keywordAuthor | Acute pancreatitis | - |
dc.subject.keywordAuthor | Autophagy | - |
dc.subject.keywordAuthor | Heat shock protein | - |
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