Interbilayer-crosslinked multilamellar vesicles as synthetic vaccines for potent humoral and cellular immune responses
- Authors
- Moon, James J.; Suh, Heikyung; Bershteyn, Anna; Stephan, Matthias T.; Liu, Hai Peng; Huang, Bonnie; Sohail, Mashaal; Luo, Samantha; Um, Soon Ho; Khant, Htet; Goodwin, Jessica T.; Ramos, Jenelyn; Chiu, Wah; Irvine, Darrell J.
- Issue Date
- Mar-2011
- Publisher
- NATURE PUBLISHING GROUP
- Citation
- NATURE MATERIALS, v.10, no.3, pp 243 - 251
- Pages
- 9
- Indexed
- SCI
SCIE
SCOPUS
- Journal Title
- NATURE MATERIALS
- Volume
- 10
- Number
- 3
- Start Page
- 243
- End Page
- 251
- URI
- https://scholarworks.korea.ac.kr/kumedicine/handle/2020.sw.kumedicine/13634
- DOI
- 10.1038/NMAT2960
- ISSN
- 1476-1122
1476-4660
- Abstract
- Vaccines based on recombinant proteins avoid the toxicity and antivector immunity associated with live vaccine ( for example, viral) vectors, but their immunogenicity is poor, particularly for CD8 C T-cell responses. Synthetic particles carrying antigens and adjuvant molecules have been developed to enhance subunit vaccines, but in general these materials have failed to elicit CD8(+) T-cell responses comparable to those for live vectors in preclinical animal models. Here, we describe interbilayer-crosslinked multilamellar vesicles formed by crosslinking headgroups of adjacent lipid bilayers within multilamellar vesicles. Interbilayer-crosslinked vesicles stably entrapped protein antigens in the vesicle core and lipid-based immunostimulatory molecules in the vesicle walls under extracellular conditions, but exhibited rapid release in the presence of endolysosomal lipases. We found that these antigen/adjuvant-carrying vesicles form an extremely potent whole-protein vaccine, eliciting endogenous T-cell and antibody responses comparable to those for the strongest vaccine vectors. These materials should enable a range of subunit vaccines and provide new possibilities for therapeutic protein delivery.
- Files in This Item
-
Go to Link
- Appears in
Collections - 2. Clinical Science > Department of Gastroenterology and Hepatology > 1. Journal Articles
Items in ScholarWorks are protected by copyright, with all rights reserved, unless otherwise indicated.