Deficiency of 15-LOX-1 Induces Radioresistance through Downregulation of MacroH2A2 in Colorectal Cancer
DC Field | Value | Language |
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dc.contributor.author | Na, Yoo Jin | - |
dc.contributor.author | Kim, Bo Ram | - |
dc.contributor.author | Kim, Jung Lim | - |
dc.contributor.author | Kang, Sanghee | - |
dc.contributor.author | Jeong, Yoon A. | - |
dc.contributor.author | Park, Seong Hye | - |
dc.contributor.author | Jo, Min Jee | - |
dc.contributor.author | Kim, Jeong-Yub | - |
dc.contributor.author | Kim, Hong Jun | - |
dc.contributor.author | Oh, Sang Cheul | - |
dc.contributor.author | Lee, Dae Hee | - |
dc.date.available | 2020-11-02T06:29:25Z | - |
dc.date.issued | 2019-11 | - |
dc.identifier.issn | 2072-6694 | - |
dc.identifier.issn | 2072-6694 | - |
dc.identifier.uri | https://scholarworks.korea.ac.kr/kumedicine/handle/2020.sw.kumedicine/1496 | - |
dc.description.abstract | Despite the importance of radiation therapy, there are few radiation-related markers available for use in clinical practice. A larger catalog of such biomarkers is required to help clinicians decide when radiotherapy should be replaced with a patient-specific treatment. Arachidonate 15-lipoxygenase (15-LOX-1) enzyme is involved in polyunsaturated fatty acid metabolism. When colorectal cancer (CRC) cells were exposed to radiation, 15-LOX-1 was upregulated. To verify whether 15-LOX-1 protects against or induces DNA damage, we irradiated sh15-LOX-1 stable cells. We found that low 15-LOX-1 is correlated with radioresistance in CRC cells. These data suggest that the presence of 15-LOX-1 can be used as a marker for radiation-induced DNA damage. Consistent with this observation, gene-set-enrichment analysis based on microarray experiments showed that UV_RESPONSE was decreased in sh15-LOX-1 cells compared to shCon cells. Moreover, we discovered that the expression of the histone H2A variant macroH2A2 was sevenfold lower in sh15-LOX-1 cells. Overall, our findings present mechanistic evidence that macroH2A2 is transcriptionally regulated by 15-LOX-1 and suppresses the DNA damage response in irradiated cells by delaying H2AX activation. | - |
dc.language | 영어 | - |
dc.language.iso | ENG | - |
dc.publisher | MDPI | - |
dc.title | Deficiency of 15-LOX-1 Induces Radioresistance through Downregulation of MacroH2A2 in Colorectal Cancer | - |
dc.type | Article | - |
dc.publisher.location | 스위스 | - |
dc.identifier.doi | 10.3390/cancers11111776 | - |
dc.identifier.scopusid | 2-s2.0-85074707376 | - |
dc.identifier.wosid | 000502290100158 | - |
dc.identifier.bibliographicCitation | CANCERS, v.11, no.11 | - |
dc.citation.title | CANCERS | - |
dc.citation.volume | 11 | - |
dc.citation.number | 11 | - |
dc.type.docType | Article | - |
dc.description.isOpenAccess | Y | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
dc.relation.journalResearchArea | Oncology | - |
dc.relation.journalWebOfScienceCategory | Oncology | - |
dc.subject.keywordPlus | EPITHELIAL-MESENCHYMAL TRANSITION | - |
dc.subject.keywordPlus | DNA-DAMAGE RESPONSE | - |
dc.subject.keywordPlus | 13-S-HYDROXYOCTADECADIENOIC ACID | - |
dc.subject.keywordPlus | RADIATION | - |
dc.subject.keywordPlus | 15-LIPOXYGENASE-1 | - |
dc.subject.keywordPlus | CYCLOOXYGENASE-2 | - |
dc.subject.keywordPlus | INHIBITION | - |
dc.subject.keywordPlus | APOPTOSIS | - |
dc.subject.keywordPlus | EXPRESSION | - |
dc.subject.keywordPlus | CELLS | - |
dc.subject.keywordAuthor | 15-LOX-1 | - |
dc.subject.keywordAuthor | colorectal cancer | - |
dc.subject.keywordAuthor | radiation | - |
dc.subject.keywordAuthor | macroH2A2 | - |
dc.subject.keywordAuthor | DNA damage | - |
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