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Cited 133 time in webofscience Cited 140 time in scopus
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Reactive oxygen species enhance differentiation of human embryonic stem cells into mesendodermal lineage

Authors
Ji A.-R.Ku S.-Y.Cho M.S.Kim Y.Y.Kim Y.J.Oh S.K.Kim S.H.Moon S.Y.Choi Y.M.
Issue Date
31-Mar-2010
Publisher
Nature Publishing Group
Keywords
Cell differentiation; Embryonic stem cells; Mitogen-activated protein kinases; Reactive oxygen species
Citation
Experimental and Molecular Medicine, v.42, no.3, pp 175 - 186
Pages
12
Indexed
SCI
SCIE
SCOPUS
KCI
Journal Title
Experimental and Molecular Medicine
Volume
42
Number
3
Start Page
175
End Page
186
URI
https://scholarworks.korea.ac.kr/kumedicine/handle/2020.sw.kumedicine/15456
DOI
10.3858/emm.2010.42.3.018
ISSN
1226-3613
2092-6413
Abstract
Recently, reactive oxygen species (ROS) have been studied as a regulator of differentiation into specific cell types in embryonic stem cells (ESCs). However, ROS role in human ESCs (hESCs) is unknown because mouse ESCs have been used mainly for most studies. Herein we suggest that ROS generation may play a critical role in differentiation of hESCs; ROS enhances differentiation of hESCs into bi-potent mesendodermal cell lineage via ROS-involved signaling pathways. In ROS-inducing conditions, expression of pluripotency markers (Oct4, Tra 1-60, Nanog, and Sox2) of hESCs was decreased, while expression of mesodermal and endodermal markers was increased. Moreover, these differentiation events of hESCs in ROS-inducing conditions were decreased by free radical scavenger treatment. hESC-derived embryoid bodies (EBs) also showed similar differentiation patterns by ROS induction. In ROS-related signaling pathway, some of the MAPKs family members in hESCs were also affected by ROS induction. p38 MAPK and AKT (protein kinases B, PKB) were inactivated significantly by buthionine sulfoximine (BSO) treatment. JNK and ERK phosphorylation levels were increased at early time of BSO treatment but not at late time point. Moreover, MAPKs family-specific inhibitors could prevent the mesendodermal differentiation of hESCs by ROS induction. Our results demonstrate that stemness and differentiation of hESCs can be regulated by environmental factors such as ROS.
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Guro Hospital (Department of Obstetrics and Gynecology, Guro Hospital)
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