In vitro response of primary human bone marrow stromal cells to recombinant human bone morphogenic protein-2 in the early and late stages of osteoblast differentiation
- Authors
- Kim, In Sook; Song, Yoon Mi; Cho, Tae Hyung; Park, Yong Doo; Lee, Kyu Back; Noh, Insup; Weber, Franz; Hwang, Soon Jung
- Issue Date
- Sep-2008
- Publisher
- WILEY
- Keywords
- alkaline phosphatase; bone morphogenic protein-2; human bone marrow stromal cells; in vitro matrix mineralization; osteoblast differentiation
- Citation
- DEVELOPMENT GROWTH & DIFFERENTIATION, v.50, no.7, pp 553 - 564
- Pages
- 12
- Indexed
- SCIE
SCOPUS
- Journal Title
- DEVELOPMENT GROWTH & DIFFERENTIATION
- Volume
- 50
- Number
- 7
- Start Page
- 553
- End Page
- 564
- URI
- https://scholarworks.korea.ac.kr/kumedicine/handle/2020.sw.kumedicine/16825
- DOI
- 10.1111/j.1440-169X.2008.01052.x
- ISSN
- 0012-1592
1440-169X
- Abstract
- A number of factors must be added to human bone marrow stromal cells (hBMSCs) in vitro to induce osteogenesis, including ascorbic acid (AA), beta-glycerophosphate (GP), and dexamethasone (Dex). Bone morphogenic protein (BMP)-2 is an osteoinductive factor that can commit stromal cells to differentiate into osteoblasts. However, it is still not clear whether the addition of BMP-2 alone in vitro can induce hBMSCs to complete osteoblast differentiation, resulting in matrix mineralization. This study compares the effects of BMP-2 and Dex, alone and combined, on the early and late stages of hBMSC differentiation. We found that BMP-2 causes a significant induction of alkaline phosphatase (ALP) activity in hBMSCs, with a transcriptional upregulation of known BMP-2-responsive genes, and the stable expression of cbfa1 in the nucleus and the regions surrounding the nucleus in the early phase of osteoblast differentiation. However, continuous treatment with BMP-2 alone at doses ranging from 100 to 300 ng/mL results in a less efficient enhancement of in vitro matrix mineralization, despite a significant induction of ALP activity at a concentration of 100 ng/mL. Our results reflect how the effects of BMP-2 on hBMSCs can vary depending on the stage of osteoblast differentiation, and highlight the need to understand the role of BMP-2 in primary hBMSCs derived from diverse sources in order to increase the efficiency of using BMP-2 in osteoinductive therapies.
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- Appears in
Collections - 1. Basic Science > Department of Biomedical Engineering > 1. Journal Articles
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