Detailed Information

Cited 21 time in webofscience Cited 23 time in scopus
Metadata Downloads

Aberrant promoter CpG islands methylation of tumor suppressor genes in cholangiocarcinoma

Authors
Uhm, Kyung-OkLee, Eun SooLee, Yun MiKim, Hyeon SooPark, Young-NyunPark, Sun-Hwa
Issue Date
Jan-2008
Publisher
Cognizant Communication Corp.
Keywords
cholangiocarcinoma; hypermethylation; methylation-specific PCR; tumor suppressor gene
Citation
Oncology Research, v.17, no.4, pp 151 - 157
Pages
7
Indexed
SCOPUS
Journal Title
Oncology Research
Volume
17
Number
4
Start Page
151
End Page
157
URI
https://scholarworks.korea.ac.kr/kumedicine/handle/2020.sw.kumedicine/17502
DOI
10.3727/096504008785114110
ISSN
0965-0407
1555-3906
Abstract
Aberrant DNA methylation of 5'-CpG islands located within gene promoters has been identified as a mechanism for transcriptional inactivation of tumor suppressor genes. To ascertain the mechanism of gene promoter hypermethylation in cholangiocarcinoma (CC), we investigated promoter methylation status of the candidate genes ID4, DLC-1, and SFRP1 in 41 CCs, 19 adjacent nontumor tissues, and 15 normal liver tissues using methylation-specific PCR (MSP). The frequencies of DNA methylation were: 57.5% (23 of 40) for ID4, 14.3% (5 of 35) for DLC-1, and 63.4% (26 of 41) for SFRP1, respectively. In contrast, a low frequency of methylation was detected in nontumor tissues. In addition, hypermethylated status of these genes was detected in three kinds of CC cell lines. Moreover, the downregulated expression of these genes in these cells was restored by treatment with 5-aza-2'-deoxycytidine, a DNA methyltransferase inhibitor. Taken together, these results suggest that abet-rant DNA methylation may contribute to the tumorigenesis of cholangiocarcinoma.
Files in This Item
There are no files associated with this item.
Appears in
Collections
1. Basic Science > Department of Anatomy > 1. Journal Articles

qrcode

Items in ScholarWorks are protected by copyright, with all rights reserved, unless otherwise indicated.

Related Researcher

Researcher Park, Sun Hwa photo

Park, Sun Hwa
College of Medicine (Department of Anatomy)
Read more

Altmetrics

Total Views & Downloads

BROWSE