Gene expression profiles during the activation of rat hepatic stellate cells evaluated by cDNA microarray
- Authors
- Woo, Sun Wook; Hwang, Kwan-Ik; Chung, Myeon-Woo; Jin, Sun Kyung; Bang, Syrie; Lee, Sung Ho; Lee, Sung Hee; Chung, Hye Joo; Sohn, Dong Hwan
- Issue Date
- Nov-2007
- Publisher
- PHARMACEUTICAL SOC KOREA
- Keywords
- hepatic stellate cell; hepatic fibrosis; gene expression; DNA microarray; DNA chip
- Citation
- ARCHIVES OF PHARMACAL RESEARCH, v.30, no.11, pp 1410 - 1418
- Pages
- 9
- Indexed
- SCIE
SCOPUS
KCI
- Journal Title
- ARCHIVES OF PHARMACAL RESEARCH
- Volume
- 30
- Number
- 11
- Start Page
- 1410
- End Page
- 1418
- URI
- https://scholarworks.korea.ac.kr/kumedicine/handle/2020.sw.kumedicine/17657
- DOI
- 10.1007/BF02977365
- ISSN
- 0253-6269
1976-3786
- Abstract
- Hepatic stellate cells (HSCs) are activated by producing potentially injurious connective tissue components during hepatic fibrosis, thereby exerting a pivotal action in the development of liver fibrogenesis. The aim of this study was to investigate differences in gene expression patterns during the activation of HSCs using complementary cDNA microarrays. HSCs were isolated from normal rat livers and cultured for 0 (3 h), 3, 5 and 7 d. RNA was extracted from cultured cells at each point. The target RNA was hybridized to gene-specific sequence probes immobilized on chips. The hybridization signal was assessed using a confocal laser scanner. Comparison of hybridization signals and patterns allows the identification of mRNAs that are expressed differentially. Statistical analysis was used to classify and cluster the genes according to their up- or downregulation. As a result, 33 upregulated early-stage and 36 upregulated late-stage gene candidates were identified. This time-based study revealed a number of newly discovered genes involved in fibrogenesis during the activation of HSCs.
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Collections - 2. Clinical Science > Department of Thoracic and Cardiovascular Surgery > 1. Journal Articles
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