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A genotype-independent real-time PCR assay for quantification of hepatitis B virus DNA

Authors
Liu Y.Hussain M.Wong S.Fung S.K.Yim H.J.Lok A.S.F.
Issue Date
2007
Citation
Journal of Clinical Microbiology, v.45, no.2, pp.553 - 558
Indexed
SCOPUS
Journal Title
Journal of Clinical Microbiology
Volume
45
Number
2
Start Page
553
End Page
558
URI
https://scholarworks.korea.ac.kr/kumedicine/handle/2020.sw.kumedicine/18404
DOI
10.1128/JCM.00709-06
ISSN
0095-1137
Abstract
Accurate quantification of hepatitis B virus (HBV) DNA levels is important for monitoring patients with chronic HBV infection and for assessing their responses to antiviral therapy. This study aimed to develop a real-time PCR assay that is sensitive and can accurately quantify a wide range of HBV DNA levels across the known HBV genotypes. An in-house real-time PCR assay using primers and a TaqMan probe in a highly conserved region of the HBV surface gene was designed. The assay was standardized against a WHO standard and validated against plasmids of HBV genotypes A through H. The linear quantification range was approximately 5 × 100 to 2.0 × 109 IU/ml. Results of samples from patients infected with HBV genotypes A through H tested using our real-time in-house PCR assay showed an excellent correlation with those of the Cobas Amplicor HBV Monitor (R2 = 0.9435) and the Cobas TaqMan HBV (R2 = 0.9873) tests. We have established a real-time PCR assay that is genotype independent and can accurately quantify a wide range of HBV DNA levels. Further studies of additional samples are ongoing to validate the genotype independence of our assay. Copyright © 2007, American Society for Microbiology. All Rights Reserved.
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Yim, Hyung Joon
Ansan Hospital (Department of Gastroenterology and Hepatology, Ansan Hospital)
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