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The translation inhibitor anisomycin induces Elk-1-mediated transcriptional activation of egr-1 through multiple mitogen-activated protein kinase pathways

Authors
Shin, Soon YoungLee, Joon HoMin, Byung WookLee, Young Han
Issue Date
31-Dec-2006
Publisher
NATURE PUBLISHING GROUP
Keywords
anisomycin; cycloheximide; early growth response protein 1; ets-domain protein Elk-1; mitogen-activated protein kinases; serum response element
Citation
EXPERIMENTAL AND MOLECULAR MEDICINE, v.38, no.6, pp.677 - 685
Indexed
SCIE
SCOPUS
KCI
Journal Title
EXPERIMENTAL AND MOLECULAR MEDICINE
Volume
38
Number
6
Start Page
677
End Page
685
URI
https://scholarworks.korea.ac.kr/kumedicine/handle/2020.sw.kumedicine/18456
DOI
10.1038/emm.2006.80
ISSN
1226-3613
Abstract
The early growth response-1 gene (egr-1) encodes a zinc-finger transcription factor Egr-1 and is rapidly inducible by a variety of extracellular stimuli. Anisomycin (ANX), a protein synthesis inhibitor, stimulates mitogen-activated protein kinase (MAPK) pathways and thereby causes a rapid induction of immediate-early response genes. We found that anisomycin treatment of U87MG glioma cells resulted in a marked, time-dependent increase in levels of Egr-1 protein. The results of Northern blot analysis and reporter gene assay of egr-1 gene promoter (Pegr-1) activity indicate that the ANX-induced increase in Egr-1 occurs at the transcriptional level. Deletion of the serum response element (SRE) in the 5'-flanking region of egr-1 gene abolished ANX-induced Pegr-1 activity. ANX induced the phosphorylation of the ERK1/2, JNK, and p38 MAPKs in a time-dependent manner and also induced transactivation of Gal4-Elk-1, suggesting that Elk-1 is involved in SRE-mediated egr-1 transcription. Transient transfection of dominant-negative constructs of MAPK pathways blocked ANX-induced P,g,l activity. Furthermore, pretreatment with specific MAPK pathway inhibitors, including the MEK inhibitor U0126, the JNK inhibitor SP600125, and the p38 kinase inhibitor SB2021190, completely inhibited ANX-inducible expression of Egr-1. Taken together, these results suggest that all three MAPK pathways play a crucial role in ANX-induced transcriptional activation Of Pegr-1 through SRE-mediated transactivation of Elk-1.
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