Induction of immunity against Hepatitis B virus surface antigen by intranasal DNA vaccination using a cationic emulsion as a mucosal gene carrier
- Authors
- Kim, Tae Woo; Chung, Hesson; Kwon, Ick Chan; Sung, Ha Chin; Kang, Tae Heung; Han, Hee Dong; Jeong, Seo Young
- Issue Date
- 31-Oct-2006
- Publisher
- SPRINGER SINGAPORE PTE LTD
- Keywords
- cationic lipid emulsion; Hepatitis B virus; mucosal DNA vaccine
- Citation
- MOLECULES AND CELLS, v.22, no.2, pp 175 - 181
- Pages
- 7
- Indexed
- SCIE
SCOPUS
KCI
- Journal Title
- MOLECULES AND CELLS
- Volume
- 22
- Number
- 2
- Start Page
- 175
- End Page
- 181
- URI
- https://scholarworks.korea.ac.kr/kumedicine/handle/2020.sw.kumedicine/18567
- ISSN
- 1016-8478
0219-1032
- Abstract
- Delivery of DNA vaccines to airway mucosa would be an ideal method for mucosal immunization. However, there have been few reports of a suitable gene delivery system. In this study we used a cationic emulsion to immunize mice via the intranasal route with pCMV-S coding for Hepatitis B virus surface antigen (HBsAg). Complexing pCMV-S with a cationic emulsion dramatically enhanced HBsAg expression in both nasal tissue and lung, and was associated with increases in the levels of HBs-specific Abs in serum and mucosal fluids, of cytotoxic T lymphocytes (CTL) in the spleen and cervical and iliac lymph nodes, and of delayed-type hypersensitivity (DTH) against HBsAg. In contrast, very weak humoral and cellular immunities were observed following immunization with naked DNA. In support of these observations, a higher proliferative response of spleenocytes was detected in the group immunized with the emulsion/pCMV-S complex than in the group immunized with naked pCMV-S. These findings may facilitate development of an emulsion-mediated gene vaccination technique for use against intracellular pathogens that invade mucosal surfaces.
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Collections - 3. Graduate School > Biomedical Research Center > 1. Journal Articles
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