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Cited 19 time in webofscience Cited 19 time in scopus
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From cloning and expression of human keratinocyte proline-rich protein (hKPRP), an epidermal marker isolated from calcium-induced differentiating keratinocytes

Authors
Lee, WHJang, SHLee, JSLee, YSeo, EYYou, KHLee, SCNam, KIKim, JMKee, SHYang, JMSeo, YJPark, JKKim, CDLee, JH
Issue Date
Nov-2005
Publisher
ELSEVIER SCIENCE INC
Keywords
calcium; epidermal differentiation; human keratinocyte proline-rich protein
Citation
JOURNAL OF INVESTIGATIVE DERMATOLOGY, v.125, no.5, pp 995 - 1000
Pages
6
Indexed
SCIE
SCOPUS
Journal Title
JOURNAL OF INVESTIGATIVE DERMATOLOGY
Volume
125
Number
5
Start Page
995
End Page
1000
URI
https://scholarworks.korea.ac.kr/kumedicine/handle/2020.sw.kumedicine/19468
DOI
10.1111/j.0022-202X.2005.23887.x
ISSN
0022-202X
1523-1747
Abstract
We isolated a human gene encoding keratinocyte proline-rich protein (hKPRP). hKPRP gene is located in the region of epidermal differentiation complex on chromosome 1q21, and its similar to 2.5 kb mRNA encodes 579 amino acid protein with high proline content (18%). The mRNA level of hKPRP was markedly increased at both 7 and 14 d after treatment with 1.2 mM calcium in cultured normal human epidermal keratinocytes. In situ hybridization demonstrated that hKPRP was expressed in upper granular layer of normal epidermis with characteristic intermittent pattern. In psoriatic lesion, hKPRP expression was increased as compared with normal skin and showed continuous pattern. Immunohistochemical analysis also confirmed the expression of hKPRP at the protein level. Western blot analysis showed that hKPRP protein of similar to 70 kDa size was significantly increased by calcium in a time-dependent manner. In mouse tissue blot assays, the expression of KPRP was detected in stomach and skin tissues, and began at 17.5 embryonic days. Additionally, hKPRP expression was detected in the periderm of human fetal skin from 16 wk estimated gestational age. Together, these results suggest that hKPRP is an epidermal marker expressed in stratified squamous epithelia and has a potential role in keratinocytes differentiation.
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