Efficient three-dimensional reconstruction of synapse with high-voltage electron microscopy
- Authors
- Lee, KJ; Park, CH; Rhyu, IJ
- Issue Date
- Apr-2005
- Publisher
- OXFORD UNIV PRESS
- Keywords
- 3-D reconstruction; HVEM; synapse; Purkinje cell; cerebellum
- Citation
- JOURNAL OF ELECTRON MICROSCOPY, v.54, no.2, pp 139 - 141
- Pages
- 3
- Indexed
- SCIE
SCOPUS
- Journal Title
- JOURNAL OF ELECTRON MICROSCOPY
- Volume
- 54
- Number
- 2
- Start Page
- 139
- End Page
- 141
- URI
- https://scholarworks.korea.ac.kr/kumedicine/handle/2020.sw.kumedicine/19768
- DOI
- 10.1093/jmicro/dfi002
- ISSN
- 0022-0744
1477-9986
- Abstract
- Three-dimensional (3-D) information on nervous tissue is essential for the understanding of brain function. Especially, 3-D synaptic analyses on serial ultrathin sections with transmission electron microscopy (TEM) have contributed to the knowledge on neural plasticity associated with various pathophysiological conditions. The 3-D reconstruction procedures, however, not only require a great amount of expertise but also include time-consuming processes. Here, we carried out computer assisted 3-D reconstruction of parallel fibre-Purkinje cell synapses based on 250 nm serial sections using high-voltage electron microscopy (HVEM). The 3-D synapse models were constructed more efficiently and rapidly compared with conventional serial TEM reconstruction. This result suggests that 3-D reconstruction with thicker sections and HVEM is a useful method to study synaptic connectivity.
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- Appears in
Collections - 1. Basic Science > Department of Anatomy > 1. Journal Articles
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