Membrane-proximal region of the carboxyl terminus of the gonadotropin-releasing hormone receptor (GnRHR) confers differential signal transduction between mammalian and nonmammalian GnRHRs
- Authors
- Oh, DY; Song, JA; Moon, JS; Moon, MJ; Kim, JI; Kim, K; Kwon, HB; Seong, JY
- Issue Date
- Mar-2005
- Publisher
- OXFORD UNIV PRESS INC
- Citation
- MOLECULAR ENDOCRINOLOGY, v.19, no.3, pp 722 - 731
- Pages
- 10
- Indexed
- SCIE
SCOPUS
- Journal Title
- MOLECULAR ENDOCRINOLOGY
- Volume
- 19
- Number
- 3
- Start Page
- 722
- End Page
- 731
- URI
- https://scholarworks.korea.ac.kr/kumedicine/handle/2020.sw.kumedicine/19818
- DOI
- 10.1210/me.2004-0220
- ISSN
- 0888-8809
1944-9917
- Abstract
- Recently, we demonstrated that the mammalian type-I GnRH receptor (GnRHR) has a high preference for the phospholipase C/protein kinase C (PLC/PKC)-linked signaling pathway, whereas non-mammalian bullfrog (bf) GnRHRs couple to both adenylate cyclase/protein kinase A (AC/PKA)- and PLC/PKC-linked signaling pathways. In the present study, using AC/PKA-specific reporter (cAMP-responsive element-luciferase) and PLC/ PKC-specific reporter (serum-responsive element-luciferase) systems, we attempted to identify the motif responsible for this difference. A deletion of the intracellular carboxyl-terminal tail ( C tail) of bfGnRHR-1 remarkably decreased its ability to induce the AC/PKA-linked signaling pathway. Further dissection of the C tail indicated that an HFRK motif in the membrane-proximal sequence of bfGnRHR-1C tail is a minimal requirement for the AC/PKA-linked signaling pathway as the addition of this motif to rat GnRHR or deletion of it from bfGnRHR-1 significantly affected the ability to induce the AC/PKA-linked signaling pathway. Deletion or addition of the HFRK motif, however, did not critically influence the PLC/PKC-linked signaling pathway. These results indicate that the HFRK motif in the membrane-proximal region confers the differential signal transduction pathways between mammalian and nonmammalian GnRHRs.
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Collections - 3. Graduate School > Biomedical Research Center > 1. Journal Articles
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