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Cited 64 time in webofscience Cited 78 time in scopus
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Vascular endothelial growth factor is increased during early stage of diabetic nephropathy in type II diabetic rats

Authors
Cha, DRKang, YSHan, SYJee, YHHan, KHHan, JYKim, YSKim, NH
Issue Date
Oct-2004
Publisher
BIOSCIENTIFICA LTD
Keywords
VEGF; type 2 diabetes; diabetic nephropathy
Citation
JOURNAL OF ENDOCRINOLOGY, v.183, no.1, pp 183 - 194
Pages
12
Indexed
SCIE
SCOPUS
Journal Title
JOURNAL OF ENDOCRINOLOGY
Volume
183
Number
1
Start Page
183
End Page
194
URI
https://scholarworks.korea.ac.kr/kumedicine/handle/2020.sw.kumedicine/20336
DOI
10.1677/joe.1.05647
ISSN
0022-0795
1479-6805
Abstract
Vascular endothelial growth factor (VEGF) has been implicated in the pathogenesis of diabetic nephropathy. We investigated serial changes of VEGF in the kidney and assessed whether glomerular and urinary VEGF levels are related to the severity of diabetic nephropathy. Furthermore, we examined the relationship between urinary VEGF levels and the urinary albumin excretion (UAE) rate in Otsuka-Long-Evans-Tokushima-Fatty (OLETF) rats. Glomerular VEGF mRNA expression and protein synthesis were evaluated by the reverse transcription-polymerase chain reaction, immunohistochemical staining and in situ hybridization. Urinary levels of VEGF were determined by enzyme-linked immunosorbent assay. UAE was significantly higher in OLETF rats than in control Long-Evans-Tokushima-Fatty (LETO) rats throughout the study period. Urinary VEGF levels were significantly higher from 25 to 37 weeks, and then gradually reduced until 55 weeks, although the levels were still higher than those in control rats. Urinary VEGF levels also showed a significant positive correlation with UAE (r=0.262, P=0.045) and serum creatinine (r=0.398, P=0.044), and were found to be independently correlated with UAE by Spearman's rank correlation. By immunohistochemical staining and in situ hybridization, VEGF was mainly detected in the podocytes in the glomeruli. Interestingly, a significant increase in VEGF mRNA expression was observed in the early period of diabetic nephropathy, and this was associated with increased urinary VEGF excretion. Thus, the overproduction of VEGF in the diabetic kidney may participate in the pathogenesis of early-stage diabetic nephropathy.
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2. Clinical Science > Department of Endocrinology and Metabolism > 1. Journal Articles
2. Clinical Science > Department of Pathology > 1. Journal Articles
2. Clinical Science > Department of Nephrology and Hypertension > 1. Journal Articles

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