Detailed Information

Cited 28 time in webofscience Cited 34 time in scopus
Metadata Downloads

Adenovirus-mediated suicide gene therapy using the human telomerase catalytic subunit (hTERT) gene promoter induced apoptosis of ovarian cancer cell line

Authors
Song, JSKim, HPYoon, WSLee, KWKim, MHKim, KTKim, HSKim, YT
Issue Date
Nov-2003
Publisher
TAYLOR & FRANCIS LTD
Keywords
telomerase; hTERT promoter; HSV-TK; adenovirus; targeted cancer gene therapy
Citation
BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY, v.67, no.11, pp 2344 - 2350
Pages
7
Indexed
SCIE
SCOPUS
Journal Title
BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY
Volume
67
Number
11
Start Page
2344
End Page
2350
URI
https://scholarworks.korea.ac.kr/kumedicine/handle/2020.sw.kumedicine/21012
DOI
10.1271/bbb.67.2344
ISSN
0916-8451
1347-6947
Abstract
Telomerase is a ribonucleoprotein complex the function of which is to add telomeric repeats (TTAGGG)(n) to chromosomal ends, and it is known to play an important role in cellular immortalization. Telomerase is highly active in most tumor cells, yet not in normal cells. As such, it may have possible applications in cancer gene therapy. Telomerase consists of two essential components, telomerase RNA template (hTR) and catalytic subunit (hTERT). hTERT is expressed only in cells and tissues positive for telomerase activity, i.e., tumor and fetal cells. We here tested the possibility of the utilization of the hTERT promoter in targeted cancer gene therapy. We cloned the hTERT promoter in the replace of the CMV promoter and sub-cloned HSV-TK gene to be controlled by hTERT gene promoter in adenovirus shuttle plasmid. Then we constructed recombinant adenovirus Ad-hT-TK, and infected them into normal and human gynecological cancer cell lines. Through these experiments, we identified the selective tumor specific cell death by Ad-hT-TK. Furthermore, FACS analysis and TUNEL assay suggests that the reduced viability is mediated through the induction of apoptosis, indicating that this approach may be a useful method for suppressing cancer growth in targeted cancer gene therapy. These results show that Ad-hT-TK could be used for gynecological cancer gene therapy.
Files in This Item
There are no files associated with this item.
Appears in
Collections
2. Clinical Science > Department of Obstetrics and Gynecology > 1. Journal Articles
4. Research institute > Allergy Immunology Center > 1. Journal Articles

qrcode

Items in ScholarWorks are protected by copyright, with all rights reserved, unless otherwise indicated.

Related Researcher

Researcher Yoon, Wonsuck photo

Yoon, Wonsuck
Research Institute (Allergy Immunology Center)
Read more

Altmetrics

Total Views & Downloads

BROWSE