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Cited 13 time in webofscience Cited 13 time in scopus
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Differentiation between reinfection and recrudescence of Helicobacter pylori strains using PCR-based restriction fragment length polymorphism analysis

Authors
Jeen, YTLee, SWKwon, SIChun, HJLee, HSSong, CWUm, SHChoi, JHKim, CDRyu, HSHyun, JH
Issue Date
Feb-2001
Publisher
YONSEI UNIV COLL MEDICINE
Keywords
Helicobacter pylori; polymorphism; restriction fragment length; duodenal ulcer; drug therapy
Citation
YONSEI MEDICAL JOURNAL, v.42, no.1, pp.41 - 45
Indexed
SCIE
SCOPUS
KCI
OTHER
Journal Title
YONSEI MEDICAL JOURNAL
Volume
42
Number
1
Start Page
41
End Page
45
URI
https://scholarworks.korea.ac.kr/kumedicine/handle/2020.sw.kumedicine/22680
DOI
10.3349/ymj.2001.42.1.41
ISSN
0513-5796
Abstract
The aim of this study was to evaluate whether PCR-based restriction fragment length polymorphism (RFLP) analysis was effective in differentiating between reinfection and recrudescence of H. pylori strains. Following a 1 - 2 week regimen of omeprazole 20 mg, amoxicillin 1.0 g, and clarithromycin 500 mg twice daily, twenty patients with duodenal ulcer were enrolled in the study. Ten patients (group I, control) were not Successfully treated, and another 10 patients (group 2) Exhibited recurrence of infection 6 - 24 months following the therapy. Follow-up diagnosis was performed by Giemsa stain and CLO test. RFLP profiles of antral and midbody biopsy specimens were compared before and after therapy. PCR products using the ureC gene were digested with restriction enzymes Hha I, Mbo I, and Hind III, and the fragments generated were analyzed by agarose gel electrophoresis. Hha 1, Mbo I, and Hind III digestion produced 13, 7, and 2 distinguishable digestion patterns, respectively. There was no difference in RFLP profiles seen before and after the therapy in 17 duodenal ulcer patients, while different RFLP profiles were discovered in 3 patients. Following treatment, one (group 2) patient differed in Mbo I, and two tone each from both groups) patients differed in Hha I and Mbo I RFLP patterns. Eight of group 2 patients showed recrudescence of previous infection and two patients had reinfection by another strain. This study supports the hypothesis that PCR-based RFLP analysis can be effective for differentiating reinfection and recrudescence of H, pylori strains following triple therapy.
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안암병원 (Department of Gastroenterology and Hepatology, Anam Hospital)
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