Detection of hepatitis A viral RNA in sera of patients with acute hepatitis A
DC Field | Value | Language |
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dc.contributor.author | Kwon, Oh Sang | - |
dc.contributor.author | Byun, Kwan Soo | - |
dc.contributor.author | Yeon, Jong Eun | - |
dc.contributor.author | Park, Sang Hoon | - |
dc.contributor.author | Kim, Jae Seon | - |
dc.contributor.author | Kim, Ju Hyun | - |
dc.contributor.author | Bak, Young Tae | - |
dc.contributor.author | Kim, Jin Ho | - |
dc.contributor.author | Lee, Chang Hong | - |
dc.date.available | 2020-11-03T22:48:47Z | - |
dc.date.issued | 2000-09 | - |
dc.identifier.issn | 0815-9319 | - |
dc.identifier.issn | 1440-1746 | - |
dc.identifier.uri | https://scholarworks.korea.ac.kr/kumedicine/handle/2020.sw.kumedicine/23102 | - |
dc.description.abstract | Background and Aims: The Detection of hepatitis A virus (HAV) is important for diagnosis and epidemiological studies of hepatitis A. The polymerase chain reaction (PCR) technique is a sensitive test to detect HAV-RNA in specimens. The aims of the present study were to clarify the detection rate of serum HAV-RNA by PCR and the natural history of HAV viraemia, and to determine the correlation between viraemia and the clinical characteristics in patients with acute hepatitis A. Methods: Hepatitis A virus RNA was tested in 74 serum samples which were serially collected from 27 patients with acute hepatitis A. A nested reverse transcription (RT)-PCR for HAV-RNA was performed with primer sets located at the VP1 region of the HAV genome and the PCR products were eletrophoresed on a 1.5% agarose gel. Results: Hepatitis A virus RNA was found in 18 of 27 (67%) patients with hepatitis A. There were no significant differences between groups positive and negative for HAV-RNA in clinical and laboratory data, except the time interval between clinical onset and initial serum sampling for RT-PCR (10 +/- 6 vs 19 +/- 14 days) and the alanine aminotransferase (ALT) level at initial serum sampling for RT-PCR (1436 +/- 1416 vs 518 +/- 432 IU/L). The mean duration of HAV viraemia was 30 +/- 19 days (range, 5-59 days). The duration of HAV viraemia and duration of abnormal ALT levels from clinical onset were positively correlated (r = 0.685, P = 0.007). Conclusion: In conclusion, HAV-RNA RT-PCR is a useful tool to detect HAV viraemia and to study the molecular epidemiology of HAV infection. (C) 2000 Blackwell Science Asia Pty Ltd. | - |
dc.format.extent | 5 | - |
dc.language | 영어 | - |
dc.language.iso | ENG | - |
dc.publisher | Blackwell Publishing Inc. | - |
dc.title | Detection of hepatitis A viral RNA in sera of patients with acute hepatitis A | - |
dc.type | Article | - |
dc.publisher.location | 미국 | - |
dc.identifier.doi | 10.1046/j.1440-1746.2000.02291.x | - |
dc.identifier.scopusid | 2-s2.0-0033784139 | - |
dc.identifier.wosid | 000089991300013 | - |
dc.identifier.bibliographicCitation | Journal of Gastroenterology and Hepatology, v.15, no.9, pp 1043 - 1047 | - |
dc.citation.title | Journal of Gastroenterology and Hepatology | - |
dc.citation.volume | 15 | - |
dc.citation.number | 9 | - |
dc.citation.startPage | 1043 | - |
dc.citation.endPage | 1047 | - |
dc.type.docType | Article | - |
dc.description.isOpenAccess | N | - |
dc.description.journalRegisteredClass | scopus | - |
dc.relation.journalResearchArea | Gastroenterology & Hepatology | - |
dc.relation.journalWebOfScienceCategory | Gastroenterology & Hepatology | - |
dc.subject.keywordPlus | POLYMERASE CHAIN-REACTION | - |
dc.subject.keywordPlus | A VIRUS | - |
dc.subject.keywordPlus | FULMINANT-HEPATITIS | - |
dc.subject.keywordPlus | INFECTION | - |
dc.subject.keywordPlus | OUTBREAK | - |
dc.subject.keywordAuthor | hepatitis A | - |
dc.subject.keywordAuthor | hepatitis A virus RNA | - |
dc.subject.keywordAuthor | serum | - |
dc.subject.keywordAuthor | viraemia | - |
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