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Cited 8 time in webofscience Cited 10 time in scopus
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The role of nuclear factor erythroid-2-related factor 2 expression in radiocontrast-induced nephropathy

Authors
Kim, Ji EunBae, So YeonAhn, Shin YoungKwon, Young JooKo, Gang Jee
Issue Date
22-Feb-2019
Publisher
NATURE PUBLISHING GROUP
Citation
SCIENTIFIC REPORTS, v.9
Indexed
SCIE
SCOPUS
Journal Title
SCIENTIFIC REPORTS
Volume
9
URI
https://scholarworks.korea.ac.kr/kumedicine/handle/2020.sw.kumedicine/2378
DOI
10.1038/s41598-019-39534-2
ISSN
2045-2322
Abstract
Radiocontrast-induced nephropathy (CIN) is the third most common cause of acute renal failure. The pathophysiology of CIN is related to tubular injury caused by oxidative stress, and nuclear factor erythroid-2-related factor 2 (Nrf2) is critical in coordinating intracellular antioxidative processes. We thus investigated the role of Nrf2 in CIN. CIN was established in mice and in NRK-52E cells via iohexol administration according to the protocols of previous studies. To determine the role of Nrf2 in CIN, Nrf2 expression was reduced in vivo using Nrf2 knockout (KO) mice (B6.129 x 1-Nfe2 l2tmlYwk/J) and in vitro with siRNA treatment targeting Nrf2. Increased Nrf2 expression was observed after iohexol treatment both in vivo and in vitro. Serum creatinine at 24 h after iohexol injection was significantly higher in KO mice than in wild-type (WT) mice. Histologic examination showed that iohexol-induced tubular vacuolization and structural disruption were aggravated in Nrf2 KO mice. Significant increases in apoptosis and F4/80(+) inflammatory cell infiltration were demonstrated in KO mice compared to WT mice. In addition, the increase in reactive oxygen species after iohexol treatment was augmented by Nrf2 inhibition both in vivo and in vitro. Nrf2 may be implicated in the pathogenesis of CIN via the modulation of antioxidant, anti-apoptotic, and anti-inflammatory processes.
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Ahn, Shin Young
Guro Hospital (Department of Nephrology and Hypertension, Guro Hospital)
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