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Cited 15 time in webofscience Cited 15 time in scopus
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Development of a Post-vitrectomy Injection of N-methyl-N-nitrosourea as a Localized Retinal Degeneration Rabbit Model

Authors
Ahn, So MinAhn, JungryulCha, SeongkwangYun, CheolminPark, Tae KwannGoo, Yong SookKim, Seong-Woo
Issue Date
Feb-2019
Publisher
KOREAN SOC BRAIN & NEURAL SCIENCE, KOREAN SOC NEURODEGENERATIVE DISEASE
Keywords
Retinal degeneration; Intravitreal injection; N-methyl-N-nitrosourea; Vitrectomy; Optical coherence tomography; Animal model
Citation
EXPERIMENTAL NEUROBIOLOGY, v.28, no.1, pp 62 - 73
Pages
12
Indexed
SCIE
SCOPUS
KCI
Journal Title
EXPERIMENTAL NEUROBIOLOGY
Volume
28
Number
1
Start Page
62
End Page
73
URI
https://scholarworks.korea.ac.kr/kumedicine/handle/2020.sw.kumedicine/2449
DOI
10.5607/en.2019.28.1.62
ISSN
1226-2560
2093-8144
Abstract
Since genetic models for retinal degeneration (RD) in animals larger than rodents have not been firmly established to date, we sought in the present study to develop a new rabbit model of drug-induced RD. First, intravitreal injection of N-methyl-N-nitrosourea (MNU) without vitrectomy in rabbits was performed with different doses. One month after injection, morphological changes in the retinas were identified with ultra-wide-field color fundus photography (FP) and fundus autofluorescence (AF) imaging as well as spectral-domain optical coherence tomography (OCT). Notably, the degree of RD was not consistently correlated with MNU dose. Then, to check the effects of vitrectomy on MNU-induced RD, the intravitreal injection of MNU after vitrectomy in rabbits was also performed with different doses. In OCT, while there were no significant changes in the retinas for injections up to 0.1 mg (i.e., sham, 0.05 mg, and 0.1 mg), outer retinal atrophy and retinal atrophy of the whole layer were observed with MNU injections of 0.3 mg and 0.5 mg, respectively. With this outcome, 0.2 mg MNU was chosen to be injected into rabbit eyes (n=10) at two weeks after vitrectomy for further study. Six weeks after injection, morphological identification with FP, AF, OCT, and histology clearly showed localized outer RD - clearly bordered non-degenerated and degenerated outer retinal area - in all rabbits. We suggest our post-vitrectomy MNU-induced RD rabbit model could be used as an interim animal model for visual prosthetics before the transition to larger animal models.
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Yun, Cheol min
Ansan Hospital (Department of Ophthalmology, Ansan Hospital)
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