Physical and Functional Interaction between 5-HT6 Receptor and Nova-1
- Authors
- Kim, Soon-Hee; Seo, Misun; Hwang, Hongik; Moon, Dong-Min; Son, Gi Hoon; Kim, Kyungjin; Rhim, Hyewhon
- Issue Date
- Feb-2019
- Publisher
- 한국뇌신경과학회
- Keywords
- Serotonin; 5-HT6 receptor; Neuro-oncological ventral antigen 1; RNA binding proteins; Neurological diseases
- Citation
- Experimental Neurobiology, v.28, no.1, pp 17 - 29
- Pages
- 13
- Indexed
- SCIE
SCOPUS
KCI
- Journal Title
- Experimental Neurobiology
- Volume
- 28
- Number
- 1
- Start Page
- 17
- End Page
- 29
- URI
- https://scholarworks.korea.ac.kr/kumedicine/handle/2020.sw.kumedicine/2450
- DOI
- 10.5607/en.2019.28.1.17
- ISSN
- 1226-2560
2093-8144
- Abstract
- 5-HT6 receptor (5-HT6R) is implicated in cognitive dysfunction, mood disorder, psychosis, and eating disorders. However, despite its significant role in regulating the brain functions, regulation of 5-HT6R at the molecular level is poorly understood. Here, using yeast two-hybrid assay, we found that human 5-HT6R directly binds to neuro-oncological ventral antigen 1 (Nova-1), a brain-enriched splicing regulator. The interaction between 5-HT6R and Nova-1 was confirmed using GST pull-down and co-immunoprecipitation assays in cell lines and rat brain. The splicing activity of Nova-1 was decreased upon overexpression of 5-HT6R, which was examined by detecting the spliced intermediates of gonadotropin-releasing hormone (GnRH), a known pre-mRNA target of Nova-1, using RT-PCR. In addition, overexpression of 5-HT6R induced the translocation of Nova-1 from the nucleus to cytoplasm, resulting in the reduced splicing activity of Nova-1. In contrast, overexpression of Nova-1 reduced the activity and the total protein levels of 5-HT6R. Taken together, these results indicate that when the expression levels of 5-HT6R or Nova-1 protein are not properly regulated, it may also deteriorate the function of the other.
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Collections - 1. Basic Science > Department of Legal Medicine > 1. Journal Articles
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