Flow Shear Stress Enhances the Proliferative Potential of Cultured Radial Glial Cells Possibly Via an Activation of Mechanosensitive Calcium Channel
- Authors
- Park, Min Gu; Jang, Heeyeong; Lee, Sang-Hoon; Lee, C. Justin
- Issue Date
- Apr-2017
- Publisher
- 한국뇌신경과학회
- Keywords
- Radial glial cell; Shear stress; Mechanosensitive ion channel
- Citation
- Experimental Neurobiology, v.26, no.2, pp 71 - 81
- Pages
- 11
- Indexed
- SCIE
SCOPUS
KCI
- Journal Title
- Experimental Neurobiology
- Volume
- 26
- Number
- 2
- Start Page
- 71
- End Page
- 81
- URI
- https://scholarworks.korea.ac.kr/kumedicine/handle/2020.sw.kumedicine/29452
- DOI
- 10.5607/en.2017.26.2.71
- ISSN
- 1226-2560
2093-8144
- Abstract
- Radial glial cells (RGCs) which function as neural stem cells are known to be non-excitable and their proliferation depends on the intracellular calcium (Ca2+) level. It has been well established that Inositol 1,4,5-trisphosphate (IP3)-mediated Ca2+ release and Ca2+ entry through various Ca2+ channels are involved in the proliferation of RGCs. Furthermore, RGCs line the ventricular wall and are exposed to a shear stress due to a physical contact with the cerebrospinal fluid (CSF). However, little is known about how the Ca2+ entry through mechanosensitive ion channels affects the proliferation of RGCs. Hence, we hypothesized that shear stress due to a flow of CSF boosts the proliferative potential of RGCs possibly via an activation of mechanosensitive Ca2+ channel during the embryonic brain development. Here, we developed a new microfluidic two-dimensional culture system to establish a link between the flow shear stress and the proliferative activity of cultured RGCs. Using this microfluidic device, we successfully visualized the artificial CSF and RGCs in direct contact and found a significant enhancement of proliferative capacity of RGCs in response to increased shear stress. To determine if there are any mechanosensitive ion channels involved, a mechanical stimulation by poking was given to individual RGCs. We found that a poking on radial glial cell induced an increase in intracellular Ca2+ level, which disappeared under the extracellular Ca2+-free condition. Our results suggest that the shear stress by CSF flow possibly activates mechanosensitive Ca2+ channels, which gives rise to a Ca2+ entry which enhances the proliferative capacity of RGCs.
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Collections - 5. Others > Others(Medicine) > 1. Journal Articles
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