In vivo assessment of the toxicity of electronic cigarettes to zebrafish (Danio rerio) embryos, following gestational exposure, in terms of mortality, developmental toxicity, and hair cell damage: Toxicity of E-cigs to zebrafish embryos
- Authors
- Chang, Young Soo; Park, S. M.; Rah, Yoon Chan; Han, E. J.; Koun, Soon il; Chang, J.; Choi, June
- Issue Date
- Jan-2021
- Publisher
- SAGE Publications
- Keywords
- Electronic cigarettes; toxicity; zebrafish; hair cell; embryonic development
- Citation
- Human and Experimental Toxicology, v.40, no.1, pp 148 - 157
- Pages
- 10
- Indexed
- SCIE
SCOPUS
- Journal Title
- Human and Experimental Toxicology
- Volume
- 40
- Number
- 1
- Start Page
- 148
- End Page
- 157
- URI
- https://scholarworks.korea.ac.kr/kumedicine/handle/2020.sw.kumedicine/33063
- DOI
- 10.1177/0960327120947785
- ISSN
- 0960-3271
1477-0903
- Abstract
- With the ban of conventional cigarettes from public spaces, electronic cigarette (E-cig) liquids have emerged as a nicotine replacement treatment for smoking cessation. However, consumers possess little knowledge of the ingredients and health effects of E-cig liquids following exposure. This study evaluated hair cell damage and developmental toxicities following gestational exposure to E-cig liquids. Zebrafish embryos were exposed to E-cig liquids at different concentrations (0.1%, 0.2%, and 0.4%). Embryonic developmental toxicity and hair cell damage was evaluated at 6 and 7 d, respectively, after fertilization. The average number of hair cells in the anterior lateral line (ALL) and posterior lateral line (PLL) following E-cig exposure was compared to that of the control. Morphological abnormalities and heart rate were evaluated. E-cig liquids significantly damaged the hair cells in the ALL, compared to the control (control; 52.85 +/- 5.29 cells, 0.1% E-cig; 49.43 +/- 7.70 cells, 0.2% E-cig; 40.68 +/- 12.00 cells, 0.4% E-cig; 32.14 +/- 20.75%; n = 29-40; p < 0.01). At high concentrations, E-cig liquids significantly damaged the hair cells in the PLL (control; 36.88 +/- 5.43 cells, 0.1% E-cig; 33.06 +/- 5.21 cells, 0.2% E-cig; 30.95 +/- 8.03 cells, 0.4% E-cig; 23.72 +/- 15.53%, n = 29-40; p < 0.01). No morphological abnormalities in body shape, somites, notochord, tail, and pectoral fin were observed; however, abnormalities were observed in the dorsal fin and heart rate at high concentrations. Thus, gestational exposure to E-cigs significantly damaged hair cells in a concentration-dependent manner and induced developmental toxicities to the dorsal fin and heart rate at high concentrations.
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Collections - 4. Research institute > Zebrafish Translational Medical Research Center > 1. Journal Articles
- 2. Clinical Science > Department of Otorhinolaryngology-Head and Neck Surgery > 1. Journal Articles
- 5. Others > Others(Medicine) > 1. Journal Articles
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