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Characterization of the cytokeratins of human colonic, pancreatic, and gastric adenocarcinoma cell lines

Authors
Rafiee P.Ho S.B.Bresalier R.S.Bloom E.J.Kim J.-H.Kim Y.S.
Issue Date
1992
Keywords
Colonic carcinoma cell line; Cytokeratin; Gastric carcinoma cell line; Pancreatic carcinoma cell line
Citation
Pancreas, v.7, no.2, pp 123 - 131
Pages
9
Indexed
SCOPUS
Journal Title
Pancreas
Volume
7
Number
2
Start Page
123
End Page
131
URI
https://scholarworks.korea.ac.kr/kumedicine/handle/2020.sw.kumedicine/39714
DOI
10.1097/00006676-199203000-00001
ISSN
0885-3177
1536-4828
Abstract
Cytokeratin-type intermediate filaments are a polygenic family of insoluble proteins that vary according to cell of origin and have been proposed as potentially useful markers of differentiation in epithelial malignancies. Be-cause gastrointestinal malignancies resemble each other in their expression of many soluble antigens, we compared the cytokeratins of seven colonic, three gastric, six pancreatic, and one duodenal carcinoma cell lines, and one colon villous adenoma cell line. Cytokeratins were characterized by one-and two-dimensional gel electrophoresis, immunoblotting, and immunocytochemistry. These cell lines expressed combinations of cytokeratins 7, 8, 18, and 19, which are typical of the “simple” epithelial pattern found in normal ductal and glan-dular tissues of the gastrointestinal tract. However, pancreatic carcinoma cell lines expressed additional cytokeratins that are normally found in stratified squamous epithelium and epidermoid (squamous cell) carcinomas. These additional cytokeratins consisted of cytokeratin 16 in all six cell, lines and cyto-keratins 4, 13, and 16 in one cell line. These results suggest that cytokeratin patterns represent stable markers that may aid in distinguishing gastrointestinal malignancies. © 1992 Raven Press, Ltd., New York.
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