Detailed Information

Cited 0 time in webofscience Cited 0 time in scopus
Metadata Downloads

Aberrant Promoter CpG Island Methylation of the hDAB2IP Gene in Colorectal Cancer

Authors
Moon, Ji WookLee, Jung OkLee, Soo KyungKim, Ji HaeYou, Ga YoungKim, JinKim, Hyeon SooPark, Sun-Hwa
Issue Date
Feb-2011
Publisher
San Lucas Medical
Keywords
Colon cancer; Hypermethylation; Methylation-specific polymerase chain reaction; Tumor suppressor gene
Citation
European journal of Clinical and Medical Oncology, v.3, no.1, pp 1 - 7
Pages
7
Indexed
SCOPUS
Journal Title
European journal of Clinical and Medical Oncology
Volume
3
Number
1
Start Page
1
End Page
7
URI
https://scholarworks.korea.ac.kr/kumedicine/handle/2020.sw.kumedicine/43702
ISSN
1759-8958
1759-8966
Abstract
Human DAB2IP (hDAB2IP), a novel GTPase-activating protein modulating Ras-mediated signaling, is a candidate tumor suppressor gene. However, promoter methylation of hDAB2IP in colorectal cancer (CRC) is poorly understood. To ascertain the mechanism of gene promoter hypermethylation in CRC cells, we investigated the promoter methylation status of hDAB2IP genes in four CRC cell lines using a methylation specific polymerase chain reaction (MS-PCR). The frequency of DNA methylation was detected in three of the four CRC cell lines. The hDAB2IP gene expression was examined and determined using a reverse transcription-PCR (RT-PCR) and immunoblot analysis in CRC cell lines. Gene expression was silenced or down-regulated in SW-480 with methylated hDAB2IP, but could be restored by treatment with 5-aza-2?-deoxycytidine, a DNA methyl-transferase inhibitor. The frequency of DNA methylation was detected in 10 of 25 colon tumors (40%), compared with 0 of 25 (0%) corresponding adjacent normal colon tissues (pB0.005). These results suggest that hDAB2IP methylation is frequently present in colon cancer, and gene silencing may play an important role in colorectal carcinogenesis.
Files in This Item
Go to Link
Appears in
Collections
2. Clinical Science > Department of Colon and Rectal Surgery > 1. Journal Articles
5. Others > Medical Science Research Management Center > 1. Journal Articles
1. Basic Science > Department of Anatomy > 1. Journal Articles
3. Graduate School > Graduate School > 1. Journal Articles

qrcode

Items in ScholarWorks are protected by copyright, with all rights reserved, unless otherwise indicated.

Related Researcher

Researcher Park, Sun Hwa photo

Park, Sun Hwa
College of Medicine (Department of Anatomy)
Read more

Altmetrics

Total Views & Downloads

BROWSE