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Cited 34 time in webofscience Cited 33 time in scopus
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Comparative analyses of plasma amyloid-β levels in heterogeneous and monomerized states by interdigitated microelectrode sensor system

Authors
Kim, YoungSooYoo, Yong KyoungKim, Hye YunRoh, Jee HoonKim, JinsikBaek, SeungyeopLee, Jinny ClaireKim, Hye JinChae, Myung-SicJeong, DahyePark, DongsungLee, SejinJang, HoChungKim, KyeonghwanLee, Jeong HoonByun, Byung HyunPark, Su YeonHa, Jeong HoLee, Kyo ChulCho, Won WooKim, Jae-SeungKoh, Jae-YoungLim, Sang MooHwang, Kyo Seon
Issue Date
Apr-2019
Publisher
American Association for the Advancement of Science
Citation
Science Advances, v.5, no.4
Indexed
SCI
SCIE
SCOPUS
Journal Title
Science Advances
Volume
5
Number
4
URI
https://scholarworks.korea.ac.kr/kumedicine/handle/2020.sw.kumedicine/52871
DOI
10.1126/sciadv.aav1388
ISSN
2375-2548
2375-2548
Abstract
Detection of amyloid-beta (A beta) aggregates contributes to the diagnosis of Alzheimer disease (AD). Plasma A beta is deemed a less invasive and more accessible hallmark of AD, as A beta can penetrate blood-brain barriers. However, correlations between biofluidic A beta concentrations and AD progression has been tenuous. Here, we introduce a diagnostic technique that compares the heterogeneous and the monomerized states of A beta in plasma. We used a small molecule, EPPS [4-(2-hydroxyethyl)-1-piperazinepropanesulfonic acid], to dissociate aggregated A beta into monomers to enhance quantification accuracy. Subsequently, A beta levels of EPPS-treated plasma were compared to those of untreated samples to minimize inter-and intraindividual variations. The interdigitated microelectrode sensor system was used to measure plasma A beta levels on a scale of 0.1 pg/ml. The implementation of this self-standard blood test resulted in substantial distinctions between patients with AD and individuals with normal cognition (NC), with selectivity and sensitivity over 90%.
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