Comparative analyses of plasma amyloid-β levels in heterogeneous and monomerized states by interdigitated microelectrode sensor system
- Authors
- Kim, YoungSoo; Yoo, Yong Kyoung; Kim, Hye Yun; Roh, Jee Hoon; Kim, Jinsik; Baek, Seungyeop; Lee, Jinny Claire; Kim, Hye Jin; Chae, Myung-Sic; Jeong, Dahye; Park, Dongsung; Lee, Sejin; Jang, HoChung; Kim, Kyeonghwan; Lee, Jeong Hoon; Byun, Byung Hyun; Park, Su Yeon; Ha, Jeong Ho; Lee, Kyo Chul; Cho, Won Woo; Kim, Jae-Seung; Koh, Jae-Young; Lim, Sang Moo; Hwang, Kyo Seon
- Issue Date
- Apr-2019
- Publisher
- American Association for the Advancement of Science
- Citation
- Science Advances, v.5, no.4
- Indexed
- SCI
SCIE
SCOPUS
- Journal Title
- Science Advances
- Volume
- 5
- Number
- 4
- URI
- https://scholarworks.korea.ac.kr/kumedicine/handle/2020.sw.kumedicine/52871
- DOI
- 10.1126/sciadv.aav1388
- ISSN
- 2375-2548
2375-2548
- Abstract
- Detection of amyloid-beta (A beta) aggregates contributes to the diagnosis of Alzheimer disease (AD). Plasma A beta is deemed a less invasive and more accessible hallmark of AD, as A beta can penetrate blood-brain barriers. However, correlations between biofluidic A beta concentrations and AD progression has been tenuous. Here, we introduce a diagnostic technique that compares the heterogeneous and the monomerized states of A beta in plasma. We used a small molecule, EPPS [4-(2-hydroxyethyl)-1-piperazinepropanesulfonic acid], to dissociate aggregated A beta into monomers to enhance quantification accuracy. Subsequently, A beta levels of EPPS-treated plasma were compared to those of untreated samples to minimize inter-and intraindividual variations. The interdigitated microelectrode sensor system was used to measure plasma A beta levels on a scale of 0.1 pg/ml. The implementation of this self-standard blood test resulted in substantial distinctions between patients with AD and individuals with normal cognition (NC), with selectivity and sensitivity over 90%.
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Collections - 1. Basic Science > Department of Physiology > 1. Journal Articles
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