Detailed Information

Cited 16 time in webofscience Cited 18 time in scopus
Metadata Downloads

The Senolytic Drug JQ1 Removes Senescent Cells via Ferroptosis

Authors
Go, SeokhyeongKang, MikyungKwon, Sung PilJung, MungyoJeon, Ok HeeKim, Byung-Soo
Issue Date
Oct-2021
Publisher
한국조직공학과 재생의학회
Keywords
Cellular senescence; Ferroptosis; JQ1; Senolytic drug
Citation
Tissue Engineering and Regenerative Medicine, v.18, no.5, pp 841 - 850
Pages
10
Indexed
SCIE
SCOPUS
KCI
Journal Title
Tissue Engineering and Regenerative Medicine
Volume
18
Number
5
Start Page
841
End Page
850
URI
https://scholarworks.korea.ac.kr/kumedicine/handle/2020.sw.kumedicine/53036
DOI
10.1007/s13770-021-00346-z
ISSN
1738-2696
2212-5469
Abstract
BACKGROUND Ferroptosis is an iron-dependent, non-apoptotic programmed cell death. Cellular senescence contributes to aging and various age-related diseases through the expression of a senescence-associated secretory phenotype (SASP). Senescent cells are often resistant to ferroptosis via increased ferritin and impaired ferritinophagy. In this study, we investigated whether treatment with JQ1 could remove senescent cells by inducing ferroptosis. METHODS Senescence of human dermal fibroblasts was induced in vitro by treating the cells with bleomycin. The senolytic effects of JQ1 were evaluated using a SA-β gal assay, annexin V analysis, cell counting kit-8 assay, and qRT-PCR. Ferroptosis following JQ1 treatment was evaluated with qRT-PCR and BODIPY staining. RESULTS At a certain range of JQ1 concentrations, JQ1 treatment reduced the viability of bleomycin-treated cells (senescent cells) but did not reduce that of untreated cells (non-senescent cells), indicating that JQ1 treatment can selectively eliminate senescent cells. JQ1 treatment also decreased SASP expression only in senescent cells. Subsequently, JQ1 treatment reduced the expression of ferroptosis-resistance genes in senescent cells. JQ1 treatment induced lipid peroxidation in senescent cells but not in non-senescent cells. CONCLUSION The data indicate that JQ1 can eliminate senescent cells via ferroptosis. This study suggests ferroptosis as a new mechanism of senolytic therapy.
Files in This Item
There are no files associated with this item.
Appears in
Collections
3. Graduate School > Biomedical Research Center > 1. Journal Articles

qrcode

Items in ScholarWorks are protected by copyright, with all rights reserved, unless otherwise indicated.

Related Researcher

Researcher Jeon, Ok hee photo

Jeon, Ok hee
Graduate School (Biomedical Research Center)
Read more

Altmetrics

Total Views & Downloads

BROWSE