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Cited 54 time in webofscience Cited 60 time in scopus
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Targeted mutagenesis in mouse cells and embryos using an enhanced prime editor

Authors
Park, Soo-JiJeong, Tae YeongShin, Seung KyunYoon, Da EunLim, Soo-YeonKim, Sol PinChoi, JungminLee, HyunjiHong, Jeong-ImAhn, JinheeSeong, Je KyungKim, Kyoungmi
Issue Date
3-Jun-2021
Publisher
BMC
Keywords
Prime editor; Igf2; Adamts20; Mouse cells and embryos; Germline transmission; Dwarf phenotype; Proximal dead sgRNA; Chromatin-modulating peptides
Citation
GENOME BIOLOGY, v.22, no.1
Indexed
SCIE
Journal Title
GENOME BIOLOGY
Volume
22
Number
1
URI
https://scholarworks.korea.ac.kr/kumedicine/handle/2020.sw.kumedicine/53775
DOI
10.1186/s13059-021-02389-w
ISSN
1474-760X
Abstract
Prime editors, novel genome-editing tools consisting of a CRISPR-Cas9 nickase and an engineered reverse transcriptase, can induce targeted mutagenesis. Nevertheless, much effort is required to optimize and improve the efficiency of prime-editing. Herein, we introduce two strategies to improve the editing efficiency using proximal dead sgRNA and chromatin-modulating peptides. We used enhanced prime-editing to generate Igf2 mutant mice with editing frequencies of up to 47% and observed germline transmission, no off-target effects, and a dwarf phenotype. This improved prime-editing method can be efficiently applied to cell research and to generate mouse models.
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3. Graduate School > Biomedical Research Center > 1. Journal Articles
1. Basic Science > Department of Physiology > 1. Journal Articles

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