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Cited 7 time in webofscience Cited 8 time in scopus
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Label-free quantitative proteomic analysis of serum extracellular vesicles differentiating patients of alcoholic and nonalcoholic fatty liver diseases

Authors
Nguyen, Huu-QuangLee, DabinKim, YeoseonBang, GeulCho, KunLee, Young-SunYeon, Jong EunLubman, David M.Kim, Jeongkwon
Issue Date
15-Aug-2021
Publisher
ELSEVIER
Keywords
Extracellular vesicles; Exosomes; Liver disease; LC-MS; MS; Proteomics
Citation
JOURNAL OF PROTEOMICS, v.245
Indexed
SCIE
SCOPUS
Journal Title
JOURNAL OF PROTEOMICS
Volume
245
URI
https://scholarworks.korea.ac.kr/kumedicine/handle/2020.sw.kumedicine/53837
DOI
10.1016/j.jprot.2021.104278
ISSN
1874-3919
Abstract
Alcoholic liver disease (ALD) and nonalcoholic fatty liver disease (NAFLD) are typically asymptomatic and slowprogressing but potentially fatal diseases that are common causes of liver cirrhosis and related complications. Exosomes are nano-sized extracellular vesicles that have been linked to various intercellular communication processes and can carry biological materials reflecting the state and severity of disease. In this study, shotgun proteomic analysis of the protein expression profiles of extracellular vesicles, including exosomes and microvesicles, enriched from human serum samples of 24 patients diagnosed with various fatty liver diseases was performed using liquid chromatography tandem mass spectrometry (LC-MS/MS) followed by protein identification and label-free quantification using the MaxQuant platform. A total of 329 proteins, including 190 previously reported exosome-specific proteins, were identified from four types of liver disease, where significant differences in protein expression were found in apolipoproteins, immunoglobulins, and other previously reported markers of liver disease. Principal component analysis of 61 proteins identified from MaxQuant analysis of the LC-MS/MS data provided a confident differentiation between ALD and NAFLD. Significance: The current investigation revealed the difference among various types of liver disease using LC-MS/ MS of exosomes enriched from human serum samples of 24 patients where the most significantly up-regulation proteins were alpha-2-macroglobulin for alcoholic hepatitis and apolipoprotein C3 for nonalcoholic fatty liver disease.
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Yeon, Jong Eun
Guro Hospital (Department of Gastroenterology and Hepatology, Guro Hospital)
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