In Situ Magnetic Control of Macroscale Nanoligand Density Regulates the Adhesion and Differentiation of Stem Cells
- Authors
- Khatua, Chandra; Min, Sunhong; Jung, Hee Joon; Shin, Jeong Eun; Li, Na; Jun, Indong; Liu, Hui-Wen; Bae, Gunhyu; Choi, Hyojun; Ko, Min Jun; Jeon, Yoo Sang; Kim, Yu Jin; Lee, Joonbum; Ko, Minji; Shim, Gyubo; Shin, Hongchul; Lee, Sangbum; Chung, Seok; Kim, Young Keun; Song, Jae-Jun; Dravid, Vinayak P.; Kang, Heemin
- Issue Date
- Jun-2020
- Publisher
- American Chemical Society
- Keywords
- charged nanoligand; spatial ligand movement; reversible ligand movement; cell adhesion; stem cell differentiation
- Citation
- Nano Letters, v.20, no.6, pp 4188 - 4196
- Pages
- 9
- Indexed
- SCIE
SCOPUS
- Journal Title
- Nano Letters
- Volume
- 20
- Number
- 6
- Start Page
- 4188
- End Page
- 4196
- URI
- https://scholarworks.korea.ac.kr/kumedicine/handle/2020.sw.kumedicine/54861
- DOI
- 10.1021/acs.nanolett.0c00559
- ISSN
- 1530-6984
1530-6992
- Abstract
- Developing materials with remote controllability of macroscale ligand presentation can mimic extracellular matrix (ECM) remodeling to regulate cellular adhesion in vivo. Herein, we designed charged mobile nanoligands with superparamagnetic nanomaterials amine-functionalized and conjugated with poly-ethylene glycol linker and negatively charged RGD ligand. We coupled negatively a charged nanoligand to a positively charged substrate by optimizing electrostatic interactions to allow reversible planar movement. We demonstrate the imaging of both macroscale and in situ nanoscale nanoligand movement by magnetically attracting charged nanoligand to manipulate macroscale ligand density. We show that in situ magnetic control of attracting charged nanoligand facilitates stem cell adhesion, both in vitro and in vivo, with reversible control. Furthermore, we unravel that in situ magnetic attraction of charged nanoligand stimulates mechanosensing- mediated differentiation of stem cells. This remote controllability of ECM-mimicking reversible ligand variations is promising for regulating diverse reparative cellular processes in vivo.
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Collections - 2. Clinical Science > Department of Otorhinolaryngology-Head and Neck Surgery > 1. Journal Articles
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