Highly efficient RNA-guided base editing in mouse embryos
- Authors
- Kim K.; Ryu S.-M.; Kim S.-T.; Baek G.; Kim D.; Lim K.; Chung E.; Kim S.; Kim J.-S.
- Issue Date
- Feb-2017
- Publisher
- Nature Publishing Group
- Citation
- Nature Biotechnology, v.35, no.5, pp 435 - 437
- Pages
- 3
- Indexed
- SCI
SCIE
SCOPUS
- Journal Title
- Nature Biotechnology
- Volume
- 35
- Number
- 5
- Start Page
- 435
- End Page
- 437
- URI
- https://scholarworks.korea.ac.kr/kumedicine/handle/2020.sw.kumedicine/5662
- DOI
- 10.1038/nbt.3816
- ISSN
- 1087-0156
1546-1696
- Abstract
- Base editors (BEs) composed of a cytidine deaminase fused to CRISPR-Cas9 convert cytidine to uridine, leading to single-base-pair substitutions in eukaryotic cells. We delivered BE mRNA or ribonucleoproteins targeting the Dmd or Tyr gene via electroporation or microinjection into mouse zygotes. F0 mice showed nonsense mutations with an efficiency of 44-57% and allelic frequencies of up to 100%, demonstrating an efficient method to generate mice with targeted point mutations. © 2017 Nature America, Inc., part of Springer Nature. All rights reserved.
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- There are no files associated with this item.
- Appears in
Collections - 1. Basic Science > Department of Physiology > 1. Journal Articles
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