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Highly efficient RNA-guided base editing in mouse embryos

Authors
Kim K.Ryu S.-M.Kim S.-T.Baek G.Kim D.Lim K.Chung E.Kim S.Kim J.-S.
Issue Date
Feb-2017
Publisher
Nature Publishing Group
Citation
Nature Biotechnology, v.35, no.5, pp 435 - 437
Pages
3
Indexed
SCI
SCIE
SCOPUS
Journal Title
Nature Biotechnology
Volume
35
Number
5
Start Page
435
End Page
437
URI
https://scholarworks.korea.ac.kr/kumedicine/handle/2020.sw.kumedicine/5662
DOI
10.1038/nbt.3816
ISSN
1087-0156
1546-1696
Abstract
Base editors (BEs) composed of a cytidine deaminase fused to CRISPR-Cas9 convert cytidine to uridine, leading to single-base-pair substitutions in eukaryotic cells. We delivered BE mRNA or ribonucleoproteins targeting the Dmd or Tyr gene via electroporation or microinjection into mouse zygotes. F0 mice showed nonsense mutations with an efficiency of 44-57% and allelic frequencies of up to 100%, demonstrating an efficient method to generate mice with targeted point mutations. © 2017 Nature America, Inc., part of Springer Nature. All rights reserved.
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