Electrophoretic Tissue Clearing and Labeling Methods for Volume Imaging of Whole Organs
- Authors
- Kim, Dai Hyun; Ahn, Hyo Hyun; Sun, Woong; Rhyu, Im Joo
- Issue Date
- Sep-2016
- Publisher
- 한국현미경학회
- Keywords
- Volume imaging; Tissue clearing technique; Single-cell resolution; Morphological analysis; Molecular labeling
- Citation
- 한국현미경학회지, v.46, no.3, pp 134 - 139
- Pages
- 6
- Indexed
- KCI
- Journal Title
- 한국현미경학회지
- Volume
- 46
- Number
- 3
- Start Page
- 134
- End Page
- 139
- URI
- https://scholarworks.korea.ac.kr/kumedicine/handle/2020.sw.kumedicine/6091
- DOI
- 10.9729/AM.2016.46.3.134
- ISSN
- 2287-5123
2287-4445
- Abstract
- Detailed structural and molecular imaging of intact organs has incurred academic interest because the associated technique is expected to provide innovative information for biological investigation and pathological diagnosis. The conventional methods for volume imaging include reconstruction of images obtained from serially sectioned tissues. This approach requires intense manual work which involves inevitable uncertainty and much time to assemble the whole image of a target organ. Recently, effective tissue clearing techniques including CLARITY and ACT-PRESTO have been reported that enables visualization of molecularly labeled structures within intact organs in three dimensions. The central principle of the methods is transformation of intact tissue into an optically transpicuous and macromolecule permeable state without loss of intrinsic structural integrity. The rapidly evolving protocols enable morphological analysis and molecular labeling of normal and pathological characteristics in large assembled biological systems with single-cell resolution. The deep tissue volume imaging will provide fundamental information about mutual interaction among adjacent structures such as connectivity of neural circuits; meso-connectome and clinically significant structural alterations according to pathologic mechanisms or treatment procedures.
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- Appears in
Collections - 1. Basic Science > Department of Anatomy > 1. Journal Articles
- 2. Clinical Science > Department of Dermatology > 1. Journal Articles
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