Diagnostic usefulness of the GenoType MTBDRplus assay for detecting drug-resistant tuberculosis using AFB smear-negative specimens with positive TB-PCR result
- Authors
- Lee Y.S.; Kang H.-R.; Lee S.-H.; Kim Y.; Kim M.-Y.; Shin J.H.; Moon J.Y.; Lee H.-K.; Park S.Y.; Mo E.-K.; Park Y.B.; Moon S.-Y.; Oh M.; Ko Y.
- Issue Date
- 2016
- Publisher
- Taylor and Francis Ltd.
- Keywords
- AFB smearnegative specimen; MTBDRplus; Mycobacterium tuberculosis
- Citation
- Infectious Diseases, v.48, no.5, pp 350 - 355
- Pages
- 6
- Indexed
- SCI
SCIE
SCOPUS
- Journal Title
- Infectious Diseases
- Volume
- 48
- Number
- 5
- Start Page
- 350
- End Page
- 355
- URI
- https://scholarworks.korea.ac.kr/kumedicine/handle/2020.sw.kumedicine/7120
- DOI
- 10.3109/23744235.2015.1122831
- ISSN
- 2374-4235
2374-4243
- Abstract
- Background The aim of this study was to evaluate the diagnostic accuracy of the GenoType MTBDRplus assay in detecting drug-resistant tuberculosis (DR-TB) by using acid-fast bacilli (AFB) smear-negative specimens with positive TB-PCR results. Methods The MTBDRplus assay was performed with 2 different categories of 117 samples, including AFB smear-positive specimens (n=53) and AFB smear-negative specimens (n=64), which exhibited positive TB-PCR results, at a single institution. The results were retrospectively compared with the results of the phenotypic drug susceptibility test (DST), for reference. Results A total of 105 tests were finally analyzed. Of these, 54 tests were conducted using AFB smear-negative specimens with positive TB-PCR results. The MTBDRplus assay for these 54 samples demonstrated a sensitivity of 100%, specificity of 98%, positive predictive value (PPV) of 75%, and negative predictive value (NPV) of 100% in detecting rifampicin resistance. With these same species, the sensitivity, specificity, PPV, and NPV values for the MTBDRplus assay were 83.3%, 97.9%, 83.3%, and 97.9%, respectively, for the detection of isoniazid resistance. The overall correlation between the MTBDRplus assay and phenotypic DST demonstrated excellent agreement for detection of rifampicin resistance (κ=0.847) and for detection of INH resistance (k=0.812), respectively. Conclusions The MTBDRplus assay can be used effectively even on AFB smear-negative specimens from TB patients, when the TB-PCR is positive. This result might help clinicians to manage patients with suspected DR-TB in difficult situations. © 2015 Society for Scandinavian Journal of Infectious Diseases.
- Files in This Item
- There are no files associated with this item.
- Appears in
Collections - 2. Clinical Science > Department of Pulmonary, Allergy, and Critical Care Medicine > 1. Journal Articles
Items in ScholarWorks are protected by copyright, with all rights reserved, unless otherwise indicated.