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Cited 6 time in webofscience Cited 8 time in scopus
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Improving Osteogenesis Activity on BMP-2-Immobilized PCL Fibers Modified by the gamma-Ray Irradiation Technique

Authors
Yun, Young-PilLee, Jae YongJeong, Won JaePark, KyeongsoonKim, Hak-JunSong, Jae-JunKim, Sung EunSong, Hae-Ryong
Issue Date
2015
Publisher
HINDAWI LTD
Citation
BIOMED RESEARCH INTERNATIONAL, v.2015
Indexed
SCIE
SCOPUS
Journal Title
BIOMED RESEARCH INTERNATIONAL
Volume
2015
URI
https://scholarworks.korea.ac.kr/kumedicine/handle/2020.sw.kumedicine/8593
DOI
10.1155/2015/302820
ISSN
2314-6133
2314-6141
Abstract
The purpose of this study was to demonstrate the ability of BMP-2-immobilized polycaprolactone (PCL) fibers modified using the gamma-ray irradiation technique to induce the osteogenic differentiation of MG-63 cells. Poly acrylic acid (AAc) was grafted onto the PCL fibers by the gamma-ray irradiation technique. BMP-2 was then subsequently immobilized onto the AAc-PCL fibers (BMP2/AAc-PCL). PCL and surface-modified PCL fibers was characterized by evaluation with a scanning electron microscope (SEM), X-ray photoelectron spectroscopy (XPS), and contact angle. The biological activity of the PCL and surface-modified PCL fibers were characterized by alkaline phosphatase (ALP) activity, calcium deposition, and them RNA expression of osteocalcin and osteopontin in MG-63 cells. Successfully grafted AAc and PCL fibers with immobilized BMP-2 were confirmed by XPS results. The results of the contact angle showed that BMP-2/AAc-PCL fibers have more hydrophilic properties in comparison to PCL fibers. The ALP activity, calcium deposition, and gene expressions of MG-63 cells grown on BMP-2/AAc-PCL fibers showed greatly induced osteogenic differentiation in comparison to the PCL fibers. In conclusion, these results demonstrated that BMP-2/AAc-PCL fibers have the potential to effectively induce the osteogenic differentiation of MG-63 cells.
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2. Clinical Science > Department of Orthopedic Surgery > 1. Journal Articles
2. Clinical Science > Department of Otorhinolaryngology-Head and Neck Surgery > 1. Journal Articles

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Song, Jae Jun
Guro Hospital (Department of Otorhinolaryngology-Head and Neck Surgery, Guro Hospital)
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