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Electron Tomography and Synapse Study

Authors
Kim, Hyun WookKim, Da SomRhyu, Im Joo
Issue Date
Sep-2014
Publisher
한국현미경학회
Keywords
Synapse; Neuroscience; Synaptic vesicle; Pooling; Postsynaptic density
Citation
한국현미경학회지, v.44, no.3, pp 83 - 87
Pages
5
Indexed
KCI
Journal Title
한국현미경학회지
Volume
44
Number
3
Start Page
83
End Page
87
URI
https://scholarworks.korea.ac.kr/kumedicine/handle/2020.sw.kumedicine/8964
DOI
10.9729/AM.2014.44.3.83
ISSN
2287-5123
2287-4445
Abstract
Electron tomography (ET) is a useful tool to investigate three-dimensional details based on virtual slices of relative thick specimen, and it requires complicated procedures consisted of image acquisition steps and image processing steps with computer program. Although the complicated step, this technique allows us to overcome some limitations of conventional transmission electron microscopy: (1) overlapping of information in the ultrathin section covering from 30 nm to 90 nm when we observe very small structures, (2) fragmentation of the information when we study larger structures over 100 nm. There are remarkable biological findings with ET, especially in the field of neuroscience, although it is not popular yet. Understanding of behavior of synaptic vesicle, active zone, pooling and fusion in the presynaptic terminal have been enhanced thanks to ET. Some sophisticated models of postsynaptic density with ET and immune labeling are introduced recently. In this review, we introduce principles, practical steps of ET and some recent researches in synapse biology.
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College of Medicine (Department of Anatomy)
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