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Cited 15 time in webofscience Cited 19 time in scopus
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Cobalt Chloride Attenuates Oxidative Stress and Inflammation through NF-kappa B Inhibition in Human Renal Proximal Tubular Epithelial Cells

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dc.contributor.authorOh, Se Won-
dc.contributor.authorLee, Yun-Mi-
dc.contributor.authorKim, Sejoong-
dc.contributor.authorChin, Ho Jun-
dc.contributor.authorChae, Dong-Wan-
dc.contributor.authorNa, Ki Young-
dc.date.available2020-11-02T17:44:28Z-
dc.date.issued2014-09-
dc.identifier.issn1011-8934-
dc.identifier.issn1598-6357-
dc.identifier.urihttps://scholarworks.korea.ac.kr/kumedicine/handle/2020.sw.kumedicine/8990-
dc.description.abstractWe evaluated the effect of cobalt chloride (CoCl2) on TNF-alpha and IFN-gamma-induced-inflammation and reactive oxygen species (ROS) in renal tubular epithelial cells (HK-2 cells). We treated HK-2 cells with CoCl2 before the administration of TNF-alpha/IFN-gamma. To regulate hemeoxygenase-1 (HO-1) expression, the cells were treated CoCl2 or HO-1 siRNA. CoCl2 reduced the generation of ROS induced by TNF-alpha/IFN-gamma. TNF-alpha/IFN-gamma-treated-cells showed an increase in the nuclear translocation of phosphorylated NF-kappa Bp65 protein, the DNA-binding activity of NF-kappa Bp50 and NF-kappa B transcriptional activity and a decrease in I kappa B alpha protein expression. These changes were restored by CoCl2. We noted an intense increase in monocyte chemoattractant protein-1 (MCP-1) and regulated on activation normal T cell expressed and secreted (RANTES) production in TNF-alpha/IFN-gamma-treated cells. We demonstrated that this effect was mediated through NF-kappa B signaling because an NF-kappa B inhibitor significantly reduced MCP-1 and RANTES production. CoCl2 effectively reduced MCP-1 and RANTES production. The expression of HO-1 was increased by CoCl2 and decreased by HO-1 siRNA. However, knockdown of HO-1 by RNA interference did not affect MCP-1 or RANTES production. We suggest that CoCl2 has a protective effect on TNF-alpha/IFN-gamma-induced inflammation through the inhibition of NF-kappa B and ROS in HK-2 cells. However, CoCl2 appears to act in an HO-1-independent manner.-
dc.language영어-
dc.language.isoENG-
dc.publisherKOREAN ACAD MEDICAL SCIENCES-
dc.titleCobalt Chloride Attenuates Oxidative Stress and Inflammation through NF-kappa B Inhibition in Human Renal Proximal Tubular Epithelial Cells-
dc.typeArticle-
dc.publisher.location대한민국-
dc.identifier.doi10.3346/jkms.2014.29.S2.S139-
dc.identifier.scopusid2-s2.0-84930976139-
dc.identifier.wosid000344320900011-
dc.identifier.bibliographicCitationJOURNAL OF KOREAN MEDICAL SCIENCE, v.29, pp S139 - S145-
dc.citation.titleJOURNAL OF KOREAN MEDICAL SCIENCE-
dc.citation.volume29-
dc.citation.startPageS139-
dc.citation.endPageS145-
dc.type.docTypeArticle-
dc.description.isOpenAccessN-
dc.description.journalRegisteredClasssci-
dc.description.journalRegisteredClassscie-
dc.description.journalRegisteredClassscopus-
dc.description.journalRegisteredClasskci-
dc.relation.journalResearchAreaGeneral & Internal Medicine-
dc.relation.journalWebOfScienceCategoryMedicine, General & Internal-
dc.subject.keywordPlusHEME OXYGENASE-1-
dc.subject.keywordPlusTNF-ALPHA-
dc.subject.keywordPlusDOWN-REGULATION-
dc.subject.keywordPlusLUNG INJURY-
dc.subject.keywordPlusKIDNEY-
dc.subject.keywordPlusEXPRESSION-
dc.subject.keywordPlusACTIVATION-
dc.subject.keywordPlusCHEMOKINE-
dc.subject.keywordPlusPATHWAYS-
dc.subject.keywordPlusDISEASES-
dc.subject.keywordAuthorCobalt Chloride-
dc.subject.keywordAuthorHemeoxygenase-1-
dc.subject.keywordAuthorInflammation-
dc.subject.keywordAuthorNuclear Factor-kappa B-
dc.subject.keywordAuthorRenal Tubular Epithelial Cells-
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Anam Hospital (Department of Nephrology and Hypertension, Anam Hospital)
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