Cobalt Chloride Attenuates Oxidative Stress and Inflammation through NF-kappa B Inhibition in Human Renal Proximal Tubular Epithelial Cells
DC Field | Value | Language |
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dc.contributor.author | Oh, Se Won | - |
dc.contributor.author | Lee, Yun-Mi | - |
dc.contributor.author | Kim, Sejoong | - |
dc.contributor.author | Chin, Ho Jun | - |
dc.contributor.author | Chae, Dong-Wan | - |
dc.contributor.author | Na, Ki Young | - |
dc.date.available | 2020-11-02T17:44:28Z | - |
dc.date.issued | 2014-09 | - |
dc.identifier.issn | 1011-8934 | - |
dc.identifier.issn | 1598-6357 | - |
dc.identifier.uri | https://scholarworks.korea.ac.kr/kumedicine/handle/2020.sw.kumedicine/8990 | - |
dc.description.abstract | We evaluated the effect of cobalt chloride (CoCl2) on TNF-alpha and IFN-gamma-induced-inflammation and reactive oxygen species (ROS) in renal tubular epithelial cells (HK-2 cells). We treated HK-2 cells with CoCl2 before the administration of TNF-alpha/IFN-gamma. To regulate hemeoxygenase-1 (HO-1) expression, the cells were treated CoCl2 or HO-1 siRNA. CoCl2 reduced the generation of ROS induced by TNF-alpha/IFN-gamma. TNF-alpha/IFN-gamma-treated-cells showed an increase in the nuclear translocation of phosphorylated NF-kappa Bp65 protein, the DNA-binding activity of NF-kappa Bp50 and NF-kappa B transcriptional activity and a decrease in I kappa B alpha protein expression. These changes were restored by CoCl2. We noted an intense increase in monocyte chemoattractant protein-1 (MCP-1) and regulated on activation normal T cell expressed and secreted (RANTES) production in TNF-alpha/IFN-gamma-treated cells. We demonstrated that this effect was mediated through NF-kappa B signaling because an NF-kappa B inhibitor significantly reduced MCP-1 and RANTES production. CoCl2 effectively reduced MCP-1 and RANTES production. The expression of HO-1 was increased by CoCl2 and decreased by HO-1 siRNA. However, knockdown of HO-1 by RNA interference did not affect MCP-1 or RANTES production. We suggest that CoCl2 has a protective effect on TNF-alpha/IFN-gamma-induced inflammation through the inhibition of NF-kappa B and ROS in HK-2 cells. However, CoCl2 appears to act in an HO-1-independent manner. | - |
dc.language | 영어 | - |
dc.language.iso | ENG | - |
dc.publisher | KOREAN ACAD MEDICAL SCIENCES | - |
dc.title | Cobalt Chloride Attenuates Oxidative Stress and Inflammation through NF-kappa B Inhibition in Human Renal Proximal Tubular Epithelial Cells | - |
dc.type | Article | - |
dc.publisher.location | 대한민국 | - |
dc.identifier.doi | 10.3346/jkms.2014.29.S2.S139 | - |
dc.identifier.scopusid | 2-s2.0-84930976139 | - |
dc.identifier.wosid | 000344320900011 | - |
dc.identifier.bibliographicCitation | JOURNAL OF KOREAN MEDICAL SCIENCE, v.29, pp S139 - S145 | - |
dc.citation.title | JOURNAL OF KOREAN MEDICAL SCIENCE | - |
dc.citation.volume | 29 | - |
dc.citation.startPage | S139 | - |
dc.citation.endPage | S145 | - |
dc.type.docType | Article | - |
dc.description.isOpenAccess | N | - |
dc.description.journalRegisteredClass | sci | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
dc.description.journalRegisteredClass | kci | - |
dc.relation.journalResearchArea | General & Internal Medicine | - |
dc.relation.journalWebOfScienceCategory | Medicine, General & Internal | - |
dc.subject.keywordPlus | HEME OXYGENASE-1 | - |
dc.subject.keywordPlus | TNF-ALPHA | - |
dc.subject.keywordPlus | DOWN-REGULATION | - |
dc.subject.keywordPlus | LUNG INJURY | - |
dc.subject.keywordPlus | KIDNEY | - |
dc.subject.keywordPlus | EXPRESSION | - |
dc.subject.keywordPlus | ACTIVATION | - |
dc.subject.keywordPlus | CHEMOKINE | - |
dc.subject.keywordPlus | PATHWAYS | - |
dc.subject.keywordPlus | DISEASES | - |
dc.subject.keywordAuthor | Cobalt Chloride | - |
dc.subject.keywordAuthor | Hemeoxygenase-1 | - |
dc.subject.keywordAuthor | Inflammation | - |
dc.subject.keywordAuthor | Nuclear Factor-kappa B | - |
dc.subject.keywordAuthor | Renal Tubular Epithelial Cells | - |
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