AMPK alpha 2 translocates into the nucleus and interacts with hnRNP H: Implications in metformin-mediated glucose uptake
- Authors
- Kim, Nami; Lee, Jung Ok; Lee, Hye Jeong; Lee, Soo Kyung; Moon, Ji Wook; Kim, Su Jin; Park, Sun Hwa; Kim, Hyeon Soo
- Issue Date
- Sep-2014
- Publisher
- ELSEVIER SCIENCE INC
- Keywords
- AMPK; Glucose uptake; hnRNP H; Phosphorylation; Translocation
- Citation
- CELLULAR SIGNALLING, v.26, no.9, pp 1800 - 1806
- Pages
- 7
- Indexed
- SCI
SCIE
SCOPUS
- Journal Title
- CELLULAR SIGNALLING
- Volume
- 26
- Number
- 9
- Start Page
- 1800
- End Page
- 1806
- URI
- https://scholarworks.korea.ac.kr/kumedicine/handle/2020.sw.kumedicine/9017
- DOI
- 10.1016/j.cellsig.2014.03.023
- ISSN
- 0898-6568
1873-3913
- Abstract
- Adenosine monophosphate (AMP)-activated protein kinase (AMPK) is a cytoplasmic protein that plays a critical role in the maintenance of energy homeostasis. However, its role in the nucleus is still largely unknown. Here, we showed that AMPK alpha 2 translocated into the nucleus during muscle differentiation. We also showed that upon treatment with 5-aminoimidazole-4-carboxy-amide-1-D-ribofuranoside (AICAR), an AMPK activator, AMPK rapidly translocated into the nucleus in rat myoblast L6 cells. On the other hand, the AMPK alpha 2 phosphorylation-defective mutant did not translocate into the nucleus. Knockdown of AMPK alpha 2 suppressed the differentiation-induced expression of myogenin, a differentiation marker. A physiological AMPK activator, metformin, also induced the translocation of AMPK alpha 2 into the nucleus. Both inhibition and knockdown of AMPK alpha 2 suppressed metformin-mediated glucose uptake. In addition, AMPK alpha 2 was shown to directly interact with the heterogeneous nuclear ribonucleoprotein H (hnRNP H). AICAR treatment increased the phosphorylation of hnRNP H. Metformin increased the interaction between AMPK alpha 2 and hnRNP H in the nucleus. Knockdown of hnRNP H blocked metformin-induced glucose uptake. In summary, these results demonstrate that AMPK alpha 2 translocates into the nucleus via phosphorylation, AMPK alpha 2 interacts with and phosphorylates hnRNP H in the nucleus, and such a protein-protein interaction modulates metformin-mediated glucose uptake. (C) 2014 Elsevier Inc. All rights reserved.
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- Appears in
Collections - 3. Graduate School > Graduate School > 1. Journal Articles
- 1. Basic Science > Department of Anatomy > 1. Journal Articles
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