Renoprotective effect of paricalcitol via a modulation of the TLR4-NF-kappa B pathway in ischemia/reperfusion-induced acute kidney injury
DC Field | Value | Language |
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dc.contributor.author | Lee, Jae-Won | - |
dc.contributor.author | Kim, Sun Chul | - |
dc.contributor.author | Ko, Yoon Sook | - |
dc.contributor.author | Lee, Hee Young | - |
dc.contributor.author | Cho, Eunjung | - |
dc.contributor.author | Kim, Myung-Gyu | - |
dc.contributor.author | Jo, Sang-Kyung | - |
dc.contributor.author | Cho, Won Yong | - |
dc.contributor.author | Kim, Hyoung Kyu | - |
dc.date.available | 2020-11-02T18:46:11Z | - |
dc.date.issued | 2014-02-07 | - |
dc.identifier.issn | 0006-291X | - |
dc.identifier.issn | 1090-2104 | - |
dc.identifier.uri | https://scholarworks.korea.ac.kr/kumedicine/handle/2020.sw.kumedicine/9578 | - |
dc.description.abstract | Background: The pathophysiology of ischemic acute kidney injury (AKI) is thought to include a complex interplay between vascular endothelial cell dysfunction, inflammation, and tubular cell damage. Several lines of evidence suggest a potential anti-inflammatory effect of vitamin D in various kidney injury models. In this study, we investigated the effect of paricalcitol, a synthetic vitamin D analog, on renal inflammation in a mouse model of ischemia/reperfusion (I/R) induced acute kidney injury (AKI). Methods: Paricalcitol was administered via intraperitoneal (IP) injection at 24 h before ischemia, and then I/R was performed through bilateral clamping of the renal pedicles. Twenty-four hours after I/R, mice were sacrificed for the evaluation of injury and inflammation. Additionally, an in vitro experiment using HK-2 cells was also performed to examine the direct effect of paricalcitol on tubular cells. Results: Pre-treatment with paricalcitol attenuated functional deterioration and histological damage in I/R induced AKI, and significantly decreased tissue neutrophil and macrophage infiltration and the levels of chemokines, the pro-inflammatory cytokine interleukin-6 (IL-6), and monocyte chemoattractant protein-1 (MCP-1). It also decreased IR-induced upregulation of Toll-like receptor 4 (TLR4), and nuclear translocation of p65 subunit of NF-kappa B. Results from the in vitro study showed pre-treatment with paricalcitol suppressed the TNE-alpha-induced depletion of cytosolic hcB in HK-2 cells. Conclusion: These results demonstrate that pre-treatment with paricalcitol has a renoprotective effect in ischemic AKI, possibly by suppressing TLR4-NF-kappa B mediated inflammation. (C) 2014 Published by Elsevier Inc. | - |
dc.format.extent | 7 | - |
dc.language | 영어 | - |
dc.language.iso | ENG | - |
dc.publisher | ACADEMIC PRESS INC ELSEVIER SCIENCE | - |
dc.title | Renoprotective effect of paricalcitol via a modulation of the TLR4-NF-kappa B pathway in ischemia/reperfusion-induced acute kidney injury | - |
dc.type | Article | - |
dc.publisher.location | 미국 | - |
dc.identifier.doi | 10.1016/j.bbrc.2014.01.005 | - |
dc.identifier.scopusid | 2-s2.0-84894586555 | - |
dc.identifier.wosid | 000331923500003 | - |
dc.identifier.bibliographicCitation | BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, v.444, no.2, pp 121 - 127 | - |
dc.citation.title | BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS | - |
dc.citation.volume | 444 | - |
dc.citation.number | 2 | - |
dc.citation.startPage | 121 | - |
dc.citation.endPage | 127 | - |
dc.type.docType | Article | - |
dc.description.isOpenAccess | N | - |
dc.description.journalRegisteredClass | sci | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
dc.relation.journalResearchArea | Biochemistry & Molecular Biology | - |
dc.relation.journalResearchArea | Biophysics | - |
dc.relation.journalWebOfScienceCategory | Biochemistry & Molecular Biology | - |
dc.relation.journalWebOfScienceCategory | Biophysics | - |
dc.subject.keywordPlus | ACUTE-RENAL-FAILURE | - |
dc.subject.keywordPlus | ISCHEMIA-REPERFUSION INJURY | - |
dc.subject.keywordPlus | FACTOR-KAPPA-B | - |
dc.subject.keywordPlus | VITAMIN-D ANALOG | - |
dc.subject.keywordPlus | INFLAMMATORY RESPONSE | - |
dc.subject.keywordPlus | ACTIVATION | - |
dc.subject.keywordPlus | RATS | - |
dc.subject.keywordPlus | EXPRESSION | - |
dc.subject.keywordPlus | DISEASE | - |
dc.subject.keywordPlus | CELLS | - |
dc.subject.keywordAuthor | Acute kidney injury | - |
dc.subject.keywordAuthor | NF-kappa B | - |
dc.subject.keywordAuthor | Paricalcitol | - |
dc.subject.keywordAuthor | Toll-like receptor 4 | - |
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