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Insulin phosphorylates tyrosine residue 464 of tub and translocates tubby into the nucleus in hircb cellsopen access

Authors
Kim, Jin WookKim, Hyeon SooKim, Sang DaePark, Jung Yul
Issue Date
Jun-2014
Publisher
Korean Endocrine Society
Keywords
Insulin; Phosphorylation; Translocation; Tubby
Citation
Endocrinology and Metabolism, v.29, no.2, pp 163 - 168
Pages
6
Indexed
SCOPUS
KCI
Journal Title
Endocrinology and Metabolism
Volume
29
Number
2
Start Page
163
End Page
168
URI
https://scholarworks.korea.ac.kr/kumedicine/handle/2020.sw.kumedicine/9900
DOI
10.3803/EnM.2014.29.2.163
ISSN
2093-596X
2093-5978
Abstract
Background: The tubby protein has a motif that might be relevant for its action in the insulin signaling pathway. Previous studies have indicated that tubby undergoes phosphorylation on tyrosine residues in response to several stimuli and is known to localize in the nucleus as well as in the plasma membrane. However, the relationship between phosphorylation and nuclear translocation is not well understood. Here, we report that insulin directly phosphorylates tubby, which translocates into the nucleus. Methods: The effects of insulin on Tubby were performed with Western blot. The immunoprecipitation and confocal microscopy were performed to prove phosphorylation and nuclear translocation. Results: Mutation study reveals that tyrosine residue 464 of tubby gene (Tub) is a phosphorylation site activated by insulin. In addition, major portions of tubby protein in the plasma membrane are translocated into the nucleus after insulin treatment. Tyrosine kinase inhibitor pretreatment blocked insulin-induced tubby translocation, suggesting that phosphorylation is important for nuclear translocation. Moreover, mutant tyrosine residue 464 did not translocate into the nucleus in respond to insulin. These findings demonstrate that insulin phosphorylates tyrosine residue 464 of Tub, and this event is important for insulin-induced tubby nuclear translocation. Conclusion: Insulin phosphorylates tyrosine residue 464 of Tub and translocates tubby into the nuclei of HIRcB cells. © 2014 Korean Endocrine Society.
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2. Clinical Science > Department of Neurosurgery > 1. Journal Articles
1. Basic Science > Department of Anatomy > 1. Journal Articles

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Kim, Sang Dae
Ansan Hospital (Department of Neurosurgery, Ansan Hospital)
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