Detailed Information

Cited 0 time in webofscience Cited 0 time in scopus
Metadata Downloads

BMP2-Mediated Silica Deposition: An Effective Strategy for Bone Mineralization

Authors
Ki, Mi-RanKim, Sung HoNguyen, Thi Khoa MySon, Ryeo GangJun, Sang HoPack, Seung Pil
Issue Date
Jan-2022
Publisher
American Chemical Society
Keywords
BMP2; bioinspired silicification; silica hybrid; bone scaffold; bone regeneration
Citation
ACS Biomaterial Science and Engineering
Indexed
SCIE
SCOPUS
Journal Title
ACS Biomaterial Science and Engineering
URI
https://scholarworks.korea.ac.kr/kumedicine/handle/2021.sw.kumedicine/55389
DOI
10.1021/acsbiomaterials.1c01095
ISSN
2373-9878
Abstract
The combined use of an osteogenic factor, such as bone morphogenetic protein 2 (BMP2), with a bone scaffold was quite functional for the reconstruction of bone defects. Although many studies using BMP2 have been done, there is still a need to develop an efficient way to apply BMP2 in the bone scaffold. Here, we reported an interesting fact that BMP2 has a silica deposition ability in the presence of silicic acid and proposed that such an ability of BMP2 can effectively immobilize and transport itself by a kind of coprecipitation of BMP2 with a silica matrix. The presence of BMP2 in the resulting silica was proved by SEM and EDS and was visualized by FITC-labeled BMP2. The delivery efficacy of BMP2 of silica-entrapped BMP2 on osteoblast differentiation and mineralization using MC3T3 E1 preosteoblast cells was evaluated in vitro. The coprecipitated BMP2 with silica exhibited osteogenesis at a low concentration that was insufficient to give an osteoinductive signal as the free form. Expectedly, the silica-entrapped BMP2 exhibited thermal stability over free BMP2. When applied to bone graft substitution, e.g., hydroxyapatite granules (HA), silica-entrapped BMP 2 laden HA (BMP2@Si/HA) showed sustained BMP2 release, whereas free BMP2 adsorbed HA by a simple dipping method (BMP2/HA) displayed a burst release of BMP2 at an initial time. In the rat critical-size calvarial defect model, BMP2@Si/HA showed better bone regeneration than BMP2/HA by about 10%. The BMP2/ silica hybrid deposited on a carrier surface via BMP2-mediated silica precipitation demonstrated an increase in the loading efficiency, a decrease in the burst release of BMP2, and an increase in bone regeneration. Taken together, the coprecipitated BMP2 with a silica matrix has the advantages of not only being able to immobilize BMP2 efficiently without compromising its function but also serving as a stable carrier for BMP2 delivery.
Files in This Item
There are no files associated with this item.
Appears in
Collections
2. Clinical Science > Department of Oral and Maxillofacial Surgery > 1. Journal Articles

qrcode

Items in ScholarWorks are protected by copyright, with all rights reserved, unless otherwise indicated.

Related Researcher

Researcher Jun, Sang Ho photo

Jun, Sang Ho
안암병원 (Department of Oral and Maxillofacial Surgery, Anam Hospital)
Read more

Altmetrics

Total Views & Downloads

BROWSE