Membrane vesicles from antibiotic-resistant Staphylococcus aureus transfer antibiotic-resistance to antibiotic-susceptible Escherichia coli
- Authors
- Lee, Ae Rin; Park, Seong Bin; Kim, Si Won; Jung, Jae Wook; Chun, Jin Hong; Kim, Jaesung; Kim, Young Rim; Lazarte, Jassy Mary S.; Jang, Ho Bin; Thompson, Kim D.; Jung, Myunghwan; Ha, Min Woo; Jung, Tae Sung
- Issue Date
- Apr-2022
- Publisher
- Blackwell Publishing Inc.
- Keywords
- antibiotic-resistant bacteria; antibiotic-susceptible bacteria; gram-negative bacteria; gram-positive bacteria; membrane vesicles (MVs); vesicle-mediated transferring of antimicrobial resistance
- Citation
- Journal of Applied Microbiology, v.132, no.4, pp 2746 - 2759
- Pages
- 14
- Indexed
- SCIE
SCOPUS
- Journal Title
- Journal of Applied Microbiology
- Volume
- 132
- Number
- 4
- Start Page
- 2746
- End Page
- 2759
- URI
- https://scholarworks.korea.ac.kr/kumedicine/handle/2021.sw.kumedicine/55449
- DOI
- 10.1111/jam.15449
- ISSN
- 1364-5072
1365-2672
- Abstract
- Aim
Bacteria naturally produce membrane vesicles (MVs), which have been shown to contribute to the spread of multi-drug resistant bacteria (MDR) by delivering antibiotic-resistant substances to antibiotic-susceptible bacteria. Here, we aim to show that MVs from Gram-positive bacteria are capable of transferring β-lactam antibiotic-resistant substances to antibiotic-sensitive Gram-negative bacteria.
Materials and Methods
MVs were collected from a methicillin-resistant strain of Staphylococcus aureus (MRSA) and vesicle-mediated fusion with antimicrobial-sensitive Escherichia coli (RC85). It was performed by exposing the bacteria to the MVs to develop antimicrobial-resistant E. coli (RC85-T).
Results
The RC85-T exhibited a higher resistance to β-lactam antibiotics compared to the parent strain. Although the secretion rates of the MVs from RC85-T and the parent strain were nearly equal, the β-lactamase activity of the MVs from RC85-T was 12-times higher than that of MVs from the parent strain, based on equivalent protein concentrations. Moreover, MVs secreted by RC85-T were able to protect β-lactam-susceptible E. coli from β-lactam antibiotic-induced growth inhibition in a dose-dependent manner.
Conclusion
MVs play a role in transferring substances from Gram-positive to Gram-negative bacteria, shown by the release of MVs from RC85-T that were able to protect β-lactam-susceptible bacteria from β-lactam antibiotics.
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- Appears in
Collections - 4. Research institute > Institute for Viral Diseases > 1. Journal Articles
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