Microfluidic electrode array chip for electrical stimulation-mediated axonal regeneration
- Authors
- Kim, Ji Woon; Choi, Yoon Young; Park, Si-Hyung; Ha, Jang Ho; Lee, Hee Uk; Kang, Taewook; Sun, Woong; Chung, Bong Geun
- Issue Date
- May-2022
- Publisher
- Royal Society of Chemistry
- Citation
- Lab on a Chip - Miniaturisation for Chemistry and Biology, v.22, no.11, pp 2122 - 2130
- Pages
- 9
- Indexed
- SCIE
SCOPUS
- Journal Title
- Lab on a Chip - Miniaturisation for Chemistry and Biology
- Volume
- 22
- Number
- 11
- Start Page
- 2122
- End Page
- 2130
- URI
- https://scholarworks.korea.ac.kr/kumedicine/handle/2021.sw.kumedicine/61059
- DOI
- 10.1039/d1lc01158h
- ISSN
- 1473-0197
1473-0189
- Abstract
- The precise manipulation of the neural stem cell (NSC)-derived neural differentiation is still challenging, and there is a technological barrier to regulate the axonal regeneration in a controlled manner. Here, we developed a microfluidic chip integrated with a microelectrode array as an axonal guidance platform. The microfluidic electrode array chip consisted of two compartments and a bridge microchannel that could isolate and guide the axons. We demonstrated that the NSCs were largely differentiated into neural cells as the electric field was applied to the microfluidic electrode array chip. We also confirmed the synergistic effects of the electrical stimulation (ES) and neurotrophic factor (NF) on axonal outgrowth. This microfluidic electrode array chip can serve as a central nervous system (CNS) model for axonal injury and regeneration. Therefore, it could be a potentially powerful tool for an in vitro model of the axonal regeneration.
- Files in This Item
- There are no files associated with this item.
- Appears in
Collections - 1. Basic Science > Department of Anatomy > 1. Journal Articles
Items in ScholarWorks are protected by copyright, with all rights reserved, unless otherwise indicated.