Establishment of a piglet model for peritoneal metastasis of ovarian canceropen access
- Lee, Eun Ji; Park, Soo Jin; Seol, Aeran; Lim, Hyunji; Park, Sumin; Ahn, Ji Yeon; Lim, Jeong Mook; Kim, Hee Seung; Korean Rotational Intraperitoneal pressurized Aerosol chemotherapy Trial Group
- Issue Date
- BioMed Central
- Large animal model; Peritoneal metastasis; Immunocompetent; Piglet; Uterine horn
- Journal of Translational Medicine, v.20, no.1
- Journal Title
- Journal of Translational Medicine
A piglet model for peritoneal metastasis (PM) of ovarian cancer was developed. It will contribute to establishing innovative chemotherapeutical and surgical strategies without any limitation on rodent models.
A total of 12 four- to five-week-old piglets of 7 to 8 kg were used. Two phases of ovarian cancer cell injections were performed with laparoscopic surgery.
In phase I trial, 5.0 × 106 SK-OV-3 cells in 0.1 ml suspension were inoculated into the omentum, peritoneum, and uterine horns of two piglets twice with a one-week interval. In the phase II trial, 5.0 × 106 SNU-008 cells in 0.1 ml suspension were injected only into uterine horns within the same time frame because tumor implantation after inoculation of SK-OV-3 cells was not observed at the omentum or peritoneum in the phase I trial. Modified peritoneal cancer index (PCI) score was used to monitor tumorigenesis up to 4 weeks after inoculation. Tumor tissues disseminated in the peritoneum 4 weeks after injection were used for histological examination with hematoxylin and eosin (H&E) and paired-box gene 8 (PAX-8) staining.
In the phase I trial, two piglets showed PM with modified PCI scores of 5 and 4 at 3 weeks after the first inoculation, which increased to 14 and 15 after 4 weeks, respectively. In the phase II trial, PM was detected in eight of ten piglets, which showed modified PCI scores of 6 to 12 at 4 weeks after the first inoculation. The overall incidence of PM from the total of 12 piglets after inoculation was 75%. Immunohistochemical H&E and PAX-8 staining confirmed metastatic tumors.
This study provides strong evidence that piglets can be employed as a model for PM by inoculating ovarian cancer cell lines from humans. Using two cell lines, the PM rate is 75%.
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- 2. Clinical Science > Department of Obstetrics and Gynecology > 1. Journal Articles
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