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Establishment of a piglet model for peritoneal metastasis of ovarian canceropen access

Authors
Lee, Eun JiPark, Soo JinSeol, AeranLim, HyunjiPark, SuminAhn, Ji YeonLim, Jeong MookKim, Hee SeungKorean Rotational Intraperitoneal pressurized Aerosol chemotherapy Trial Group
Issue Date
Jul-2022
Publisher
BioMed Central
Keywords
Large animal model; Peritoneal metastasis; Immunocompetent; Piglet; Uterine horn
Citation
Journal of Translational Medicine, v.20, no.1
Indexed
SCIE
SCOPUS
Journal Title
Journal of Translational Medicine
Volume
20
Number
1
URI
https://scholarworks.korea.ac.kr/kumedicine/handle/2021.sw.kumedicine/61294
DOI
10.1186/s12967-022-03533-1
Abstract
Background A piglet model for peritoneal metastasis (PM) of ovarian cancer was developed. It will contribute to establishing innovative chemotherapeutical and surgical strategies without any limitation on rodent models. Methods A total of 12 four- to five-week-old piglets of 7 to 8 kg were used. Two phases of ovarian cancer cell injections were performed with laparoscopic surgery. In phase I trial, 5.0 × 106 SK-OV-3 cells in 0.1 ml suspension were inoculated into the omentum, peritoneum, and uterine horns of two piglets twice with a one-week interval. In the phase II trial, 5.0 × 106 SNU-008 cells in 0.1 ml suspension were injected only into uterine horns within the same time frame because tumor implantation after inoculation of SK-OV-3 cells was not observed at the omentum or peritoneum in the phase I trial. Modified peritoneal cancer index (PCI) score was used to monitor tumorigenesis up to 4 weeks after inoculation. Tumor tissues disseminated in the peritoneum 4 weeks after injection were used for histological examination with hematoxylin and eosin (H&E) and paired-box gene 8 (PAX-8) staining. Results In the phase I trial, two piglets showed PM with modified PCI scores of 5 and 4 at 3 weeks after the first inoculation, which increased to 14 and 15 after 4 weeks, respectively. In the phase II trial, PM was detected in eight of ten piglets, which showed modified PCI scores of 6 to 12 at 4 weeks after the first inoculation. The overall incidence of PM from the total of 12 piglets after inoculation was 75%. Immunohistochemical H&E and PAX-8 staining confirmed metastatic tumors. Conclusions This study provides strong evidence that piglets can be employed as a model for PM by inoculating ovarian cancer cell lines from humans. Using two cell lines, the PM rate is 75%.
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2. Clinical Science > Department of Obstetrics and Gynecology > 1. Journal Articles

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