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Establishment of a piglet model for peritoneal metastasis of ovarian cancer

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dc.contributor.authorLee, Eun Ji-
dc.contributor.authorPark, Soo Jin-
dc.contributor.authorSeol, Aeran-
dc.contributor.authorLim, Hyunji-
dc.contributor.authorPark, Sumin-
dc.contributor.authorAhn, Ji Yeon-
dc.contributor.authorLim, Jeong Mook-
dc.contributor.authorKim, Hee Seung-
dc.contributor.authorKorean Rotational Intraperitoneal pressurized Aerosol chemotherapy Trial Group-
dc.date.accessioned2022-08-10T00:40:28Z-
dc.date.available2022-08-10T00:40:28Z-
dc.date.issued2022-07-
dc.identifier.issn1479-5876-
dc.identifier.urihttps://scholarworks.korea.ac.kr/kumedicine/handle/2021.sw.kumedicine/61294-
dc.description.abstractBackground A piglet model for peritoneal metastasis (PM) of ovarian cancer was developed. It will contribute to establishing innovative chemotherapeutical and surgical strategies without any limitation on rodent models. Methods A total of 12 four- to five-week-old piglets of 7 to 8 kg were used. Two phases of ovarian cancer cell injections were performed with laparoscopic surgery. In phase I trial, 5.0 × 106 SK-OV-3 cells in 0.1 ml suspension were inoculated into the omentum, peritoneum, and uterine horns of two piglets twice with a one-week interval. In the phase II trial, 5.0 × 106 SNU-008 cells in 0.1 ml suspension were injected only into uterine horns within the same time frame because tumor implantation after inoculation of SK-OV-3 cells was not observed at the omentum or peritoneum in the phase I trial. Modified peritoneal cancer index (PCI) score was used to monitor tumorigenesis up to 4 weeks after inoculation. Tumor tissues disseminated in the peritoneum 4 weeks after injection were used for histological examination with hematoxylin and eosin (H&E) and paired-box gene 8 (PAX-8) staining. Results In the phase I trial, two piglets showed PM with modified PCI scores of 5 and 4 at 3 weeks after the first inoculation, which increased to 14 and 15 after 4 weeks, respectively. In the phase II trial, PM was detected in eight of ten piglets, which showed modified PCI scores of 6 to 12 at 4 weeks after the first inoculation. The overall incidence of PM from the total of 12 piglets after inoculation was 75%. Immunohistochemical H&E and PAX-8 staining confirmed metastatic tumors. Conclusions This study provides strong evidence that piglets can be employed as a model for PM by inoculating ovarian cancer cell lines from humans. Using two cell lines, the PM rate is 75%.-
dc.language영어-
dc.language.isoENG-
dc.publisherBioMed Central-
dc.titleEstablishment of a piglet model for peritoneal metastasis of ovarian cancer-
dc.typeArticle-
dc.publisher.location영국-
dc.identifier.doi10.1186/s12967-022-03533-1-
dc.identifier.scopusid2-s2.0-85134507493-
dc.identifier.wosid000828689900001-
dc.identifier.bibliographicCitationJournal of Translational Medicine, v.20, no.1-
dc.citation.titleJournal of Translational Medicine-
dc.citation.volume20-
dc.citation.number1-
dc.type.docTypeArticle-
dc.description.isOpenAccessY-
dc.description.journalRegisteredClassscie-
dc.description.journalRegisteredClassscopus-
dc.relation.journalResearchAreaResearch & Experimental Medicine-
dc.relation.journalWebOfScienceCategoryMedicine, Research & Experimental-
dc.subject.keywordPlusLARGE ANIMAL-MODELS-
dc.subject.keywordPlusLYMPHOCYTE SUBSETS-
dc.subject.keywordPlusCARCINOMATOSIS-
dc.subject.keywordPlusTUMOR-
dc.subject.keywordPlusCHEMOTHERAPY-
dc.subject.keywordPlusMARKER-
dc.subject.keywordPlusBLOOD-
dc.subject.keywordPlusPIGS-
dc.subject.keywordPlusPAX8-
dc.subject.keywordAuthorLarge animal model-
dc.subject.keywordAuthorPeritoneal metastasis-
dc.subject.keywordAuthorImmunocompetent-
dc.subject.keywordAuthorPiglet-
dc.subject.keywordAuthorUterine horn-
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Seol, Aeran
Anam Hospital (Department of Obstetrics and Gynecology, Anam Hospital)
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