Humoral and Cellular Immune Responses to Vector, Mix-and-Match, or mRNA Vaccines against SARS-CoV-2 and the Relationship between the Two Immune Responsesopen access
- Authors
- Nam, Minjeong; Yun, Seung Gyu; Kim, Sang-wook; Kim, Chris Gunwoo; Cha, Jae Hyun; Lee, Cheonghwa; Kang, Seunghyuk; Park, Seul Gi; Kim, Sun Bean; Lee, Ki-Byung; Chung, You-Seung; Nam, Myung-Hyun; Lee, Chang Kyu; Cho, Yunjung
- Issue Date
- Jul-2022
- Publisher
- American Society for Microbiology
- Keywords
- cellular immune responses; humoral immune responses; vector vaccine; mix-and-match vaccine; mRNA vaccine
- Citation
- Microbiology spectrum, v.10, no.4
- Indexed
- SCIE
SCOPUS
- Journal Title
- Microbiology spectrum
- Volume
- 10
- Number
- 4
- URI
- https://scholarworks.korea.ac.kr/kumedicine/handle/2021.sw.kumedicine/61362
- DOI
- 10.1128/spectrum.02495-21
- ISSN
- 2165-0497
2165-0497
- Abstract
- We investigated how differences in age, sex, or vaccine type can affect humoral and cellular immune responses after vaccination with vector (ChAdOx1 nCoV-19), mix-and-match (first, ChAdOx1 nCoV-19, and second, BNT162b2), or mRNA (BNT162b2 or mRNA-1273) vaccines against severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2). Venous blood was collected from 573 subjects (vector, 396; mix-and-match, 96; and mRNA, 81) before the first vaccination (T0), 7 to 8 weeks (vector) or 3 to 4 weeks (mRNA) after the first vaccination (T1), and 3 to 4 weeks after the second vaccination (T2). The humoral and cellular immune responses were evaluated using Elecsys anti-SARS-CoV-2 (Roche), Alinity SARS-CoV-2 IgG II Quant (Abbott), cPass SARS-CoV-2 neutralization antibody detection (GenScript), and QuantiFERON SARS-CoV-2 (Qiagen) kits. At T1, the levels of the receptor-binding domain antibodies (RBD Ab) and neutralizing antibodies (NAb) decreased with aging, but interferon gamma release (IGR) levels increased. The RBD Ab, NAb, and IGR levels were higher in females than in males at T1 and T2. The NAb levels were higher in the mix-and-match and mRNA vaccine groups than in the vector vaccine group at T2. The RBD Ab and IGR levels were higher in the mRNA vaccine group than in the vector or mix-and-match vaccine groups at T2. The optimal cutoffs for RBD Ab and NAb, which were used to determine the presence of T cell responses, were 5.7 binding antibody units per milliliter (BAU mL−1) and 12.0 IU mL−1, respectively. Age, sex, and vaccine type affected the humoral and cellular immune responses, and T cell responses could be estimated from RBD Ab and NAb levels.
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- Appears in
Collections - 2. Clinical Science > Department of Infectious Diseases > 1. Journal Articles
- 2. Clinical Science > Department of Laboratory Medicine > 1. Journal Articles
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