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Crosstalk between mucosal microbiota, host gene expression, and sociomedical factors in the progression of colorectal canceropen access

Authors
Kim, NamjooGim, Jeong-AnLee, Beom JaeChoi, Byung ilYoon, Hee SookKim, Seung HanJoo, Moon KyungPark, Jong-JaeKim, Chungyeul
Issue Date
Aug-2022
Publisher
Nature Publishing Group
Citation
Scientific Reports, v.12, no.1
Indexed
SCIE
SCOPUS
Journal Title
Scientific Reports
Volume
12
Number
1
URI
https://scholarworks.korea.ac.kr/kumedicine/handle/2021.sw.kumedicine/61368
DOI
10.1038/s41598-022-17823-7
Abstract
Various omics-based biomarkers related to the occurrence, progression, and prognosis of colorectal cancer (CRC) have been identified. In this study, we attempted to identify gut microbiome-based biomarkers and detect their association with host gene expression in the initiation and progression of CRC by integrating analysis of the gut mucosal metagenome, RNA sequencing, and sociomedical factors. We performed metagenome and RNA sequencing on colonic mucosa samples from 13 patients with advanced CRC (ACRC), 10 patients with high-risk adenoma (HRA), and 7 normal control (NC) individuals. All participants completed a questionnaire on sociomedical factors. The interaction and correlation between changes in the microbiome and gene expression were assessed using bioinformatic analysis. When comparing HRA and NC samples, which can be considered to represent the process of tumor initiation, 28 genes and five microbiome species were analyzed with correlation plots. When comparing ACRC and HRA samples, which can be considered to represent the progression of CRC, seven bacterial species and 21 genes were analyzed. When comparing ACRC and NC samples, 16 genes and five bacterial species were analyzed, and four correlation plots were generated. A network visualizing the relationship between bacterial and host gene expression in the initiation and progression of CRC indicated that Clostridium spiroforme and Tyzzerella nexilis were hub bacteria in the development and progression of CRC. Our study revealed the interactions of and correlation between the colonic mucosal microbiome and host gene expression to identify potential roles of the microbiome in the initiation and progression of CRC. Our results provide gut microbiome-based biomarkers that may be potential diagnostic markers and therapeutic targets in patients with CRC.
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4. Research institute > Institute for Trauma Research > 1. Journal Articles
2. Clinical Science > Department of Gastroenterology and Hepatology > 1. Journal Articles
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Kim, Chung yeul
Guro Hospital (Department of Pathology, Guro Hospital)
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